Antibacterial effect of synthetic peptide LyeTxI and LyeTxI/ß-Cyclodextrin association compound against planktonic and multispecies biofilms of periodontal pathogens

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dc.creatorEdison Andrés Cruz Olivo
dc.creatorDaniel Santos
dc.creatorMaria Elena de Lima
dc.creatorVera Lúcia dos Santos
dc.creatorRubén Dario Sinisterra Millán
dc.creatorMaría Esperanza Cortés Segura
dc.date.accessioned2023-11-22T13:17:11Z
dc.date.accessioned2025-09-09T00:46:06Z
dc.date.available2023-11-22T13:17:11Z
dc.date.issued2017-06
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.description.sponsorshipOutra Agência
dc.identifier.doihttps://doi.org/10.1902/jop.2016.160438
dc.identifier.issn1943-3670
dc.identifier.urihttps://hdl.handle.net/1843/61249
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofJournal of Periodontology
dc.rightsAcesso Restrito
dc.subjectCiclodextrinas
dc.subjectBiofilme
dc.subjectDoença periodontal
dc.subjectAgentes antibacterianos
dc.subjectPeriodontite
dc.subjectMicroscopia eletrônica de varredura
dc.subjectFluorimetria
dc.subject.otherAntimicrobial cationic peptides
dc.subject.otherBiofilms
dc.subject.otherCyclodextrins
dc.subject.otherFusobacterium
dc.subject.otherPeriodontitis
dc.subject.otherPorphyromonas
dc.titleAntibacterial effect of synthetic peptide LyeTxI and LyeTxI/ß-Cyclodextrin association compound against planktonic and multispecies biofilms of periodontal pathogens
dc.typeArtigo de periódico
local.citation.epagee96
local.citation.issue6
local.citation.spagee88
local.citation.volume88
local.description.resumoBackground: Antimicrobial peptides (AMPs) have shown rapid and potent effect against planktonic bacteria. However, control of periodontopathic biofilms is a challenge even for AMPs. Thus, the present study evaluates in vitro antimicrobial activity of synthetic peptide LyeTxI and association compound LyeTxI/β-cyclodextrin (βCD) against multispecies biofilms. Methods: Sensibility to LyeTxI and LyeTxI/βCD was determined for planktonic Gram-negative periodontopathogens. Time-kill kinetic assay was performed at minimum inhibitory concentrations (MICs) in all planktonic strains. Multispecies biofilms were grown on pegs using a biofilm device and studied by scanning electron microscopy at 2, 5, and 10 days. Minimal biofilm eradication concentration (MBEC) was determined for 2- and 4-day multispecies biofilms. Metabolic activity of biofilms was determined by fluorometry study. Results: Biofilms showed reproducible cell density on pegs of the biofilm device. LyeTxI and LyeTxI/βCD were active against all strains tested at concentrations ≤62.5 μg/mL. Kinetic assays showed rapid bactericidal effect of LyeTxI against all periodontopathogens. MBECs of LyeTxI and LyeTxI/βCD against multispecies 2-day biofilms were two-fold higher than MICs of cells shed from biofilms. LyeTxI was able to reduce multispecies 2-day metabolic activity by 90%. Multispecies 4-day biofilms were tolerant to all agents tested. Conclusions: LyeTxI and LyeTxI/βCD are active against periodontopathic bacteria, showing rapid bactericidal effect and may be used to prevent biofilm development. In the future, AMPs could be therapeutic tools for treatment of periodontitis.
local.identifier.orcidhttps://orcid.org/0000-0001-7416-3775
local.identifier.orcidhttps://orcid.org/0000-0001-6185-4032
local.identifier.orcidhttps://orcid.org/0000-0001-5815-5652
local.identifier.orcidhttps://orcid.org/0000-0001-7656-1849
local.identifier.orcidhttps://orcid.org/0000-0002-0560-8491
local.publisher.countryBrasil
local.publisher.departmentFAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORA
local.publisher.departmentICB - DEPARTAMENTO DE BIOQUÍMICA E IMUNOLOGIA
local.publisher.departmentICB - DEPARTAMENTO DE MICROBIOLOGIA
local.publisher.departmentICX - DEPARTAMENTO DE QUÍMICA
local.publisher.initialsUFMG
local.url.externahttps://aap.onlinelibrary.wiley.com/doi/10.1902/jop.2016.160438

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