Targeting Wistar rat as a model for studying benign, premalignant and malignant lesions of the prostate

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dc.creatorGabriel Henrique Campolina Silva
dc.creatorHipácia Werneck Gomes
dc.creatorBruna T. Maria
dc.creatorMaria C. Barata
dc.creatorMaría J. Torres
dc.creatorHector Contreras
dc.creatorGermán A.B. Mahecha
dc.creatorCleida Aparecida de Oliveira
dc.date.accessioned2023-07-14T20:20:29Z
dc.date.accessioned2025-09-08T22:59:41Z
dc.date.available2023-07-14T20:20:29Z
dc.date.issued2020
dc.identifier.doihttps://doi.org/10.1016/j.lfs.2019.117149
dc.identifier.issn1879-0631
dc.identifier.urihttps://hdl.handle.net/1843/56281
dc.languagepor
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofLife Sciences
dc.rightsAcesso Aberto
dc.subjectProstata
dc.subjectAdenocarcinoma
dc.subjectEnvelhecimento
dc.subject.otherProstate lesions
dc.subject.otherAdenocarcinoma
dc.subject.otherAging
dc.subject.otherAndrogen: estrogen imbalance
dc.subject.otherWistar rat
dc.titleTargeting Wistar rat as a model for studying benign, premalignant and malignant lesions of the prostate
dc.typeArtigo de periódico
local.citation.epage13
local.citation.issue1
local.citation.spage1
local.citation.volume242
local.description.resumoAims: The purpose of this study was to describe a suitable experimental model for studying aging-related prostate disorders including cancer. Materials and methods: 12-month old Wistarratswere keptincontrol conditions (n=12)or treated(n= 16)for 6 months with Silastic implants filled with testosterone (T) and estradiol (E2). After the experiment period (at 18 months of age), animals were euthanized and the prostate and other organs were harvested, dissected, weighed, and processed for morphological, ultrastructural and molecular analyses. Key findings: We demonstrated that male rats of Wistar strain nicely recapitulate the carcinogenesis process taking place in the aging prostate through the arising of benign, precancerous and malignant lesions, and above all yields a modest incidence of spontaneous PCa (~36%). Moreover, our results highlight that 100% incidence of PCa and precancerous lesions such as prostatic intraepithelial neoplasia and proliferative inflammatory atrophy were achieved in this ratstrain after T+ E2 treatment, without changing the broad spectrum of changes that naturally emerge in the prostate at advanced ages. Such enhancement of precancerous lesions and tumors was linked to a decreased expression of E-cadherin andβ-catenin in parallel with an increase in Vimentin and Ncadherin, hallmark modifications of epithelial-mesenchymal transition. Significance: Our findings provide solid evidence that aged Wistar rats may be an excellent model for studies regarding human prostate biology and related disorders including cancer.
local.identifier.orcidhttps://orcid.org/0000-0002-4473-3340
local.identifier.orcidhttps://orcid.org/0000-0001-5243-8272
local.identifier.orcidhttps://orcid.org/0000-0003-4012-2662
local.identifier.orcidhttps://orcid.org/0000-0003-2907-0945
local.identifier.orcidhttps://orcid.org/0000-0001-9610-7846
local.publisher.countryBrasil
local.publisher.departmentICB - DEPARTAMENTO DE MORFOLOGIA
local.publisher.initialsUFMG
local.url.externahttps://www.sciencedirect.com/science/article/pii/S002432051931077X

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