Subcutaneous model for the study of dengue virus infection in immune competent mice

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dc.creatorBeatriz Senra Santos
dc.creatorNatalia Lima Pessoa
dc.creatorNatalia Lucinda
dc.creatorGustavo Cardoso de Oliveira
dc.creatorThais Souza Silva
dc.creatorKetyllen Reis Andrade
dc.creatorBruno Galvão Filho
dc.creatorMarcele Neves Rocha
dc.creatorAlexandre Vieira Machado
dc.creatorPedro Augusto Alves
dc.creatorÉrica Alessandra Rocha Alves
dc.creatorErna Geessien Kroon
dc.creatorMarco Antônio Campos
dc.date.accessioned2025-02-10T21:58:19Z
dc.date.accessioned2025-09-08T23:00:00Z
dc.date.available2025-02-10T21:58:19Z
dc.date.issued2018-05-24
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.4236/jbm.2018.65011
dc.identifier.issn2327-509X
dc.identifier.urihttps://hdl.handle.net/1843/79844
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofJournal of Biosciences and Medicines
dc.rightsAcesso Aberto
dc.subjectCitocinas
dc.subjectDengue
dc.subject.otherSubcutaneous Murine Model
dc.subject.otherDengue Disease
dc.subject.otherImmune Competent Mouse
dc.subject.otherCytokines
dc.subject.otherInnate Immune Response
dc.titleSubcutaneous model for the study of dengue virus infection in immune competent mice
dc.typeArtigo de periódico
local.citation.epage110
local.citation.issue5
local.citation.spage97
local.citation.volume6
local.description.resumoVarious mouse models to study dengue have been described by different authors, some of them using immunodeficient or some using humanized mice. Our group reported previously a deadly murine model, which used the intracranial inoculum of highly virulent Dengue virus (DENV) in immune competent mouse. Here we present a model of immune competent mouse (C57BL/6), infected subcutaneously by the same highly virulent DENV (DENV3 genotype I). In this immunocompetent systemic mice model, the cytokine levels and hematological parameters such as total and differential leukocyte and platelets counts, together with weight loss, were considered important monitoring parameters, allowing a better understanding of the systemic human disease. Mice were inoculated subcutaneously and evaluated by the percentage weight variation as well as the clinical signs. Hematological parameters and cytokines levels were measured and viral titration in brain tissue or serum neutralization was performed to confirm mice infection. The subcutaneously DENV inoculated mice showed weight loss after infection, but they did not show any other clinical signs. The leukocytes and platelets decreased after subcutaneous inoculation. The cytokines TNF alpha and IFN gamma increased after infection in mice. The subcutaneous model provided scope for improved understanding of the dengue pathogenesis, as well as possible mechanism for protection to subsequent mouse infected by intracranial route in mice. This model could be used to study the vertebrate immune response and evaluation of drugs or vaccine against dengue virus.
local.identifier.orcidhttps://orcid.org/0000-0002-4284-2674
local.identifier.orcidhttps://orcid.org/0000-0002-9166-3111
local.identifier.orcidhttps://orcid.org/0000-0001-8278-2445
local.identifier.orcidhttps://orcid.org/0000-0001-7130-6961
local.identifier.orcidhttps://orcid.org/0000-0003-1506-4093
local.identifier.orcidhttps://orcid.org/0000-0003-2721-3826
local.publisher.countryBrasil
local.publisher.departmentICB - DEPARTAMENTO DE MICROBIOLOGIA
local.publisher.initialsUFMG
local.url.externahttps://www.scirp.org/pdf/JBM_2018052315022911.pdf

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