Maternal vitamin D deficiency affects the morphology and function of glycolytic muscle in adult offspring rats

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dc.creatorNatany Garcia Reis
dc.creatorNeusa Maria Zanon
dc.creatorTatiana E. Koike
dc.creatorAudrei R. Santos
dc.creatorElen H. Miyabara
dc.creatorIsis do Carmo Kettelhut
dc.creatorLuiz Carlos Navegantes
dc.creatorAna Paula Assis
dc.creatorNatalia Lauterbach
dc.creatorDawit Albieiro Pinheiro Gonçalves
dc.creatorWilian de Assis Silveira
dc.creatorHenrique J. N. Morgan
dc.creatorRafael Valentim
dc.creatorLucas F. Almeida
dc.creatorLilian do Carmo Heck
dc.date.accessioned2024-02-27T16:21:23Z
dc.date.accessioned2025-09-09T00:46:19Z
dc.date.available2024-02-27T16:21:23Z
dc.date.issued2022-05-18
dc.description.sponsorshipFAPESP - Fundação de Amparo à Pesquisa do Estado de São Paulo
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.1002/jcsm.12986
dc.identifier.issn2190-6009
dc.identifier.urihttps://hdl.handle.net/1843/64800
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofJournal of Cachexia, Sarcopenia and Muscle
dc.rightsAcesso Aberto
dc.subjectVitamina D
dc.subjectFeto
dc.subjectEsqueleto
dc.subjectMúsculos
dc.subjectHipertrofia
dc.subject.otherFetal programming
dc.subject.otherVitamin D
dc.subject.otherSkeletal muscle
dc.subject.otherMuscle development
dc.subject.otherHypertrophy
dc.titleMaternal vitamin D deficiency affects the morphology and function of glycolytic muscle in adult offspring rats
dc.typeArtigo de periódico
local.citation.epage2187
local.citation.spage2175
local.citation.volume13
local.description.resumoBackground Fetal stage is a critical developmental window for the skeletal muscle, but little information is available about the impact of maternal vitamin D (Vit. D) deficiency (VDD) on offspring lean mass development in the adult life of male and female animals. Methods Female rats (Wistar Hannover) were fed either a control (1000 IU Vit. D3/kg) or a VDD diet (0 IU Vit. D3/kg) for 6 weeks and during gestation and lactation. At weaning, male and female offspring were randomly separated and received a standard diet up to 180 days old. Results Vitamin D deficiency induced muscle atrophy in the male (M-VDD) offspring at the end of weaning, an effect that was reverted along the time. Following 180 days, fast-twitch skeletal muscles [extensor digitorum longus (EDL)] from the M-VDD showed a decrease (20%; P < 0.05) in the number of total fibres but an increase in the cross-sectional area of IIB (17%; P < 0.05), IIA (19%; P < 0.05) and IIAX (21%; P < 0.05) fibres. The fibre hypertrophy was associated with the higher protein levels of MyoD (73%; P < 0.05) and myogenin (55% %; P < 0.05) and in the number of satellite cells (128.8 ± 14 vs. 91 ± 7.6 nuclei Pax7 + in the M-CTRL; P < 0.05). M-VDD increased time to fatigue during ex vivo contractions of EDL muscles and showed an increase in the phosphorylation levels of IGF-1/insulin receptor and their downstream targets related to anabolic processes and myogenic activation, including Ser 473Akt and Ser 21/9GSK-3β. In such muscles, maternal VDD induced a compensatory increase in the content of calcitriol (two-fold; P < 0.05) and CYP27B1 (58%; P < 0.05), a metabolizing enzyme that converts calcidiol to calcitriol. Interestingly, most morphological and biochemical changes found in EDL were not observed in slow-twitch skeletal muscles (soleus) from the M-VDD group as well as in both EDL and soleus muscles from the female offspring. Conclusions These data show that maternal VDD selectively affects the development of type-II muscle fibres in male offspring rats but not in female offspring rats and suggest that the enhancement of their size and fatigue resistance in fast-twitch skeletal muscle (EDL) is probably due to a compensatory increase in the muscle content of Vit. D in the adult age.
local.identifier.orcidhttps://orcid.org/0000-0003-0391-7255
local.identifier.orcidhttps://orcid.org/0000-0002-5738-9374
local.identifier.orcidhttps://orcid.org/0000-0001-6933-6598
local.identifier.orcidhttps://orcid.org/0000-0003-2621-3330
local.identifier.orcidhttps://orcid.org/0000-0002-6171-7940
local.identifier.orcidhttps://orcid.org/0000-0001-5062-4493
local.identifier.orcidhttps://orcid.org/0000-0002-2352-6449
local.identifier.orcidhttps://orcid.org/0000-0002-5731-1219
local.identifier.orcidhttps://orcid.org/0000-0002-9423-9965
local.identifier.orcidhttps://orcid.org/0000-0002-9366-2593
local.identifier.orcidhttps://orcid.org/0000-0002-2325-9571
local.identifier.orcidhttps://orcid.org/0000-0002-9034-5357
local.identifier.orcidhttps://orcid.org/0000-0002-9870-9469
local.publisher.countryBrasil
local.publisher.departmentEEF - DEPARTAMENTO DE EDUCAÇÃO FÍSICA
local.publisher.departmentEEFFTO - ESCOLA DE EDUCAÇÃO FISICA, FISIOTERAPIA E TERAPIA OCUPACIONAL
local.publisher.initialsUFMG
local.url.externahttps://onlinelibrary.wiley.com/doi/10.1002/jcsm.12986

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