Schoolchildren’s hand hygiene microbiota: a pilot study
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Universidade Federal de Minas Gerais
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Artigo de periódico
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Membros da banca
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Objective: This study aimed to show schoolchildren the vari
ety of microorganisms present before and after hand hygiene,
and to make them aware of the transmission and autoinoculation
of pathogenic microorganisms. Study design: This study was
approved by ethical committee and is part of the "Projeto Experi
mentando Ci^ encia." A total of 27 volunteers from 5 different
public schools, aged from 11 to 15 years, participated in this
study. A microbiota sample was collected using sterile swab and
Sabouraud dextrose agar plates before and after hand hygiene
orientation (World Health Organization [WHO] technique 1).
After 1 week, plates were shown and explained to the participat
ing children. Different materials were used for hand hygiene in
each school. Results: A total of 35% used water and paper towels
to sanitize the hands; 32% used water, soap and paper towels;
30%used only water; and 3% used soap and water. A larger vari
ety and number of colonies were found before sanitization than
after, even in the group that washed hands only with water. Fungi
such as Penicillium, Aspergillus, Cladosporium, and nonsporu
lated types, as well as bacteria, were identified on the samples.
One week after the procedure, teachers reported children
changed their hand hygiene habits. Conclusion: Visualization of
fungi and bacteria culture improved schoolchildren hand
hygiene.
Abstract
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Bacteria, Hand hygiene
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https://www.sciencedirect.com/science/article/pii/S2212440320309287