Comparison of five methods of extraction of staphylococcus aureus dna for molecular detection by pcr

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dc.creatorMaristela Oliveiralara
dc.creatorThabata Coaglio Lucas
dc.creatorEvanguedes Kalapothakis
dc.creatorRonaldo Luis Thomasini
dc.creatorCarla Jorge Machado
dc.date.accessioned2024-01-23T19:47:50Z
dc.date.accessioned2025-09-08T23:21:50Z
dc.date.available2024-01-23T19:47:50Z
dc.date.issued2018
dc.format.mimetypepdf
dc.identifier.doi10.1590/0037-8682-0352-2017
dc.identifier.issn16789849
dc.identifier.urihttps://hdl.handle.net/1843/63266
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofRevista da Sociedade Brasileira de Medicina Tropical
dc.rightsAcesso Aberto
dc.subjectPolymerase Chain Reaction
dc.subjectDNA, Bacterial
dc.subjectCross Infection
dc.subject.otherPolymerase Chain Reaction
dc.subject.otherDNA, Bacterial
dc.subject.otherDNA extraction
dc.subject.otherNosocomial infection
dc.titleComparison of five methods of extraction of staphylococcus aureus dna for molecular detection by pcr
dc.typeArtigo de periódico
local.citation.epage532
local.citation.issue4
local.citation.spage528
local.citation.volume51
local.description.resumoIntroduction: Molecular techniques for the detection of pathogens have been shown to be effective diagnostic tools with high sensitivity and short turnaround times. Methods: This study compared five Staphylococcus aureus DNA extraction methods for detection by the polymerase chain reaction. Results: The concentration and purity of the extracted DNA showed that the methods did not yield DNA of significant quality. However, most protocols yielded 100% positivity, even with low DNA concentrations. Conclusions: Although one protocol seemed more efficient than the others, PCR was sensitive enough to allow for detection of S. aureus with all the protocols
local.publisher.countryBrasil
local.publisher.departmentMED - DEPARTAMENTO DE MEDICINA PREVENTIVA SOCIAL
local.publisher.initialsUFMG
local.url.externahttps://doi.org/10.1590/0037-8682-0352-2017

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