Efeitos do uso de fibrina rica em plaquetas aplicada sobre colorrafia em ratos

Abstract

Introduction: The most feared complication in intestinal surgery is anastomotic dehiscence, which is closely related to poor healing and can occur in 2% to 15% of operated patients. This complication has a high morbidity and mortality rate, interferes with surgical results, increases hospital costs and, in oncological cases, worsens the prognosis. Several factors can influence the healing of the colic anastomosis, such as poor nutritional status. The application of biomaterials has been extensively studied, such as platelet-rich fibrin (PRF), which has important properties in healing and tissue restoration. The possible beneficial effects of PRF on intestinal healing motivated this study. Objective: To evaluate the effects of PRF on the healing of intestinal sutures in rats. Material and method: 40 rats were distributed into 4 groups. Two groups were treated with standard food and considered nourished (I and II). Two other groups were treated with cornmeal, a food low in protein, and considered malnourished (III and IV). All animals underwent cecotomy and cecorrhaphy. Groups II and IV had the sutures overlapped with a human PRF membrane. The following parameters were evaluated: animal weight, death, rupture site, rupture pressure, collagen dosage and reticulin dosage in the suture line. Results: the use of PRF did not influence deaths, rupture pressure or rupture location in the fed animals. For malnourished animals, a significant difference was observed in relation to the rupture site (p=0.038) and reticulin dosage (p=0.040), when PRF was used. There was no difference in relation to burst pressures, but there was a tendency for an increase in collagen dosage in malnourished animals that received the PRF membrane. Conclusion: The use of PRF did not influence the healing of nourished rats. In the group of malnourished animals, its use favored healing and allowed the colic suture to be more resistant than the colic wall during the rupture pressure tests and evaluation of the rupture site. Keywords: 1-Colic suture. 2-Platelet-rich fibrin (PRF). 3-Healing. 4-Breaking pressure. 5-Dehiscence.