Different culture conditions applied to in vitro shoot multiplication of two Eucalyptus benthamii explant sources

Página do item simplificado
dc.creatorNatalia Pimentel Esposito-Polesi
dc.creatorLeandro Silva de Oliveira
dc.creatorFrancisco José Benedini Baccarin
dc.creatorCristina Vieira de Almeida
dc.creatorMarcílio de Almeida
dc.date.accessioned2023-05-23T20:01:07Z
dc.date.accessioned2025-09-08T23:47:01Z
dc.date.available2023-05-23T20:01:07Z
dc.date.issued2020
dc.identifier.doihttps://doi.org/10.1007/s11676-018-0816-1
dc.identifier.issn1007-662X
dc.identifier.urihttps://hdl.handle.net/1843/53822
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofJournal of Forestry Research
dc.rightsAcesso Restrito
dc.subjectCelulas e tecidos vegetais
dc.subjectSacarose
dc.subjectBrotos (Plantas)
dc.subjectEucalipto
dc.subject.otherPlant tissue culture
dc.subject.otherExplant sources
dc.subject.otherCulture media
dc.subject.otherSucrose
dc.subject.otherpH
dc.titleDifferent culture conditions applied to in vitro shoot multiplication of two Eucalyptus benthamii explant sources
dc.typeArtigo de periódico
local.citation.epage869
local.citation.issue3
local.citation.spage857
local.citation.volume31
local.description.resumoEucalyptus adult material requires more successive subcultures in the in vitro multiplication phase for increased vigor and cellular activity. This study evaluated the endophytic manifestation and shoot multiplication of one 13-year-old Eucalyptus benthamii clone under different culture conditions and used canopy branches (CB) and trunk base material as explant sources. The culture media were wood plant medium (WPM), Murashige and Skoog medium (MS) and JADS (Correia and co-authors medium). Based on the results of the initial multiplication experiment, further tests examined sucrose concentrations and pH. Morphophysiology, dry mass production, endophytic manifestation and histochemical were determined. Explant sources responded differently to MS and JADS media, but the WPM medium promoted homogeneous development. The responses were similar for both explant sources when sucrose concentrations varied. Shoots died in the absence of sucrose, showed high oxidation at 60 g L-1 and optimal development at 30 g L-1. Endophytes were more evident for shoots from the CB origin. Explant sources responded distinctively to treatment due to physiological and intrinsic genetic factors. Therefore, explant sources, different culture media, sucrose concentration and pH may determine micropropagation success and influence the presence and/ or intensity of endophytic manifestation.
local.publisher.countryBrasil
local.publisher.departmentICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS
local.publisher.initialsUFMG
local.url.externahttps://link.springer.com/article/10.1007/s11676-018-0816-1

Arquivos

Licença do pacote

Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
License.txt
Tamanho:
1.99 KB
Formato:
Plain Text
Descrição:
Baixar

Coleções

Artigo de Periódico

Logo UFMG