Altered umbilical cord blood nutrient levels, placental cell turnover and transporter expression in human term pregnancies conceived by intracytoplasmic sperm injection (icsi)

Página do item simplificado
dc.creatorEnrrico Bloise
dc.creatorFlavia F. Bloise
dc.creatorStephen G. Matthews
dc.creatorKristin L. Connor
dc.creatorTania M. Ortiga-Carvalho
dc.creatorJair R. S. Braga
dc.creatorCherley B. V. Andrade
dc.creatorGuinever E. Imperio
dc.creatorLilian M. Martinelli
dc.creatorRoberto A. Antunes
dc.creatorKarina R. Silva
dc.creatorCristiana Buzelin Nunes
dc.creatorLuigi Cobellis
dc.date.accessioned2023-05-15T20:03:09Z
dc.date.accessioned2025-09-09T00:50:01Z
dc.date.available2023-05-15T20:03:09Z
dc.date.issued2021
dc.format.mimetypepdf
dc.identifier.doi10.3390/nu13082587
dc.identifier.issn20726643
dc.identifier.urihttps://hdl.handle.net/1843/53388
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofNutrients
dc.rightsAcesso Aberto
dc.subjectTécnicas de Reprodução Assistida
dc.subjectAminoácidos
dc.subjectCitrulina
dc.subjectGlucose
dc.subjectPlacenta
dc.subject.otherAssisted reproductive technology (ART)
dc.subject.otherIntracytoplasmic sperm injection (ICSI)
dc.subject.otherAmino acids
dc.subject.otherCitruline
dc.subject.otherGlucose
dc.subject.otherLipids
dc.subject.otherFree fatty acids (FFA
dc.subject.otherPlacenta
dc.subject.otherProliferation
dc.subject.otherApoptosis
dc.subject.otherGLUT1
dc.subject.otherSNAT2
dc.subject.otherP-gp and BCRP
dc.titleAltered umbilical cord blood nutrient levels, placental cell turnover and transporter expression in human term pregnancies conceived by intracytoplasmic sperm injection (icsi)
dc.typeArtigo de periódico
local.citation.epage18
local.citation.issue2587
local.citation.spage1
local.citation.volume13
local.description.resumoAssisted reproductive technologies (ART) may increase risk for abnormal placental development, preterm delivery and low birthweight. We investigated placental morphology, transporter expression and paired maternal/umbilical fasting blood nutrient levels in human term pregnancies conceived naturally (n = 10) or by intracytoplasmic sperm injection (ICSI; n = 11). Maternal and umbilical vein blood from singleton term (>37 weeks) C-section pregnancies were assessed for levels of free amino acids, glucose, free fatty acids (FFA), cholesterol, high density lipoprotein (HDL), low density lipoprotein (LDL), very low-density lipoprotein (VLDL) and triglycerides. We quantified placental expression of GLUT1 (glucose), SNAT2 (amino acids), P-glycoprotein (P-gp) and breast cancer resistance protein (BCRP) (drug) transporters, and placental morphology and pathology. Following ICSI, placental SNAT2 protein expression was downregulated and umbilical cord blood levels of citrulline were increased, while FFA levels were decreased at term (p < 0.05). Placental proliferation and apoptotic rates were increased in ICSI placentae (p < 0.05). No changes in maternal blood nutrient levels, placental GLUT1, P-gp and BCRP expression, or placental histopathology were observed. In term pregnancies, ICSI impairs placental SNAT2 transporter expression and cell turnover, and alters umbilical vein levels of specific nutrients without changing placental morphology. These may represent mechanisms through which ICSI impacts pregnancy outcomes and programs disease risk trajectories in offspring across the life course.
local.identifier.orcidhttps://orcid.org/0000-0002-5266-6870
local.publisher.countryBrasil
local.publisher.departmentICB - DEPARTAMENTO DE MORFOLOGIA
local.publisher.departmentMED - DEPARTAMENTO DE ANATOMIA PATOLÓGICA E MEDICINA LEGAL
local.publisher.initialsUFMG
local.url.externahttps://doi:10.3390/nu13082587

Arquivos

Pacote original

Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
Altered Umbilical Cord Blood Nutrient Levels, Placental Cell pdfa.pdf
Tamanho:
1.88 MB
Formato:
Adobe Portable Document Format
Baixar

Licença do pacote

Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
License.txt
Tamanho:
1.99 KB
Formato:
Plain Text
Descrição:
Baixar

Coleções

Artigo de Periódico

Logo UFMG