Effects of leaf and seed alcoholic extracts from of Annona crassiflora Mart. (Annonaceae) in mice

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dc.creatorOtávio Cardoso Filho
dc.creatorDeborah de Farias Lelis
dc.creatorMatheus Ferreira Inácio
dc.creatorLudmilla Regina Souza
dc.creatorRenata Danielle Ribeiro
dc.creatorRaynald Lício Ferreira Brandão Silva
dc.creatorLuís Paulo Oliveira
dc.creatorRenê Ferreira Costa
dc.creatorEduardo Robson Duarte
dc.creatorFranciellen Morais Costa
dc.date.accessioned2023-03-03T11:25:48Z
dc.date.accessioned2025-09-09T01:08:27Z
dc.date.available2023-03-03T11:25:48Z
dc.date.issued2018-09-29
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.identifier.issn2230-9926
dc.identifier.urihttps://hdl.handle.net/1843/50620
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.rightsAcesso Aberto
dc.subjectAnonacea
dc.subjectToxicidade - Testes
dc.subjectCamundongos
dc.titleEffects of leaf and seed alcoholic extracts from of Annona crassiflora Mart. (Annonaceae) in mice
dc.typeArtigo de periódico
local.citation.epage22831
local.citation.issue9
local.citation.spage22826
local.citation.volume8
local.description.resumoAnnona crassiflora Mart. is a species of the cerrado biome in the family Annonaceae. It is a vegetable with therapeutic potential. However, it should be emphasized that its toxicity when consumed in large doses remains unknown, and clinical studies are strongly suggested for this purpose. The objective of this study was to verify the toxicological effect of leaf and seed extracts of Annona crassiflora Mart. (Annonaceae) in mice. The plant was collected in were collected in cerrado areas in Montes Claros, Minas Gerais, Brazil. Healthy leaves and seeds were selected and dried in a forced air circulating dryer at 40ºC for 72 h. Ethanolic extract (EE) was obtained by maceration of the dried leaves in absolute ethanol. The extracts were filtered and subsequently dehydrated at 40ºC for 48 h. The chromatographic profile was performed using an HPLC system. To evaluate the toxicological effects, 18 Swiss mice, were divided into three groups, composed of three males and three females each as follows: 22 μL PBS (C), 22 μL leaf extract (LE), 22 μL seed extract (SE) that were administered by means of gavage at cumulative doses for four days at the following concentrations: day one, 12.5mg/kg; day two, 25.0mg/kg; day three 50mg/kg and the fourth day, 100mg/kg. To assess the toxicological effects, physical aspects, behavior, and body weight of animals were monitored daily. On the fifth day the mice were euthanized and peripheral blood and target tissues were collected. ALT plasma level was measured. Liver, spleen, heart, and kidney samples were collected, formalin-fisxed, paraffin-embedded, and submited to histopathological analysis. Statistical analysis was performed using Student t tests and ANOVA. The Bonferroni test was applied as post hoc. Valeus of p < 0.05 were considered as statistically significant. Our findings showed that A. crassiflora Mart. extract is rich in flavonoids and flavones. No clinical signs or symptoms that had suggested toxicity were observed in all animals. However LE and SE animals showed decreased body weight, increased size and wight for liver and spleen organs. Moreover, ALT levels were increased in experimental animals. Histological analysis showed morphological findings of discrete hyperemia in liver and spleen tissues of animals treated with SE. In conclusion, the present study evidenced potential toxic effects of A.crassiflora Mart. leaf and seed extract notably in liver and spleen organs. More prospective studies are needed to better understand the cytotoxic effects of A. crassiflora.
local.publisher.countryBrasil
local.publisher.departmentICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS
local.publisher.initialsUFMG
local.url.externahttps://www.journalijdr.com/effects-leaf-and-seed-alcoholic-extracts-annona-crassiflora-mart-annonaceae-mice

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