The long noncoding RNA KIAA0125 is aberrantly expressed in ameloblastomas

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dc.creatorSílvia Ferreira de Sousa
dc.creatorMarina Gonçalves Diniz
dc.creatorJosiane Alves França
dc.creatorFabrício de Almeida Souza Vilas Boas
dc.creatorFabrício Tinôco Alvim de Souza
dc.creatorGeorge Adrian Calin
dc.creatorRicardo Santiago Gomez
dc.creatorCarolina Cavaliéri Gomes
dc.date.accessioned2025-07-28T16:52:17Z
dc.date.accessioned2025-09-08T23:02:34Z
dc.date.available2025-07-28T16:52:17Z
dc.date.issued2019-07-01
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.1158/1538-7445.AM2019-1811
dc.identifier.issn1538-7445
dc.identifier.urihttps://hdl.handle.net/1843/83886
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofProceedings of the American Association for Cancer Research Annual Meeting 2019
dc.rightsAcesso Restrito
dc.subjectGene expression
dc.subjectChromosomes, human, pair 14
dc.subjectGenes
dc.subjectAdenomatoid tumor
dc.subjectValidation study
dc.subjectTherapeutics
dc.titleThe long noncoding RNA KIAA0125 is aberrantly expressed in ameloblastomas
dc.typeArtigo de evento
local.citation.epage1811
local.citation.issue13
local.citation.spage1811
local.description.resumoLong noncoding RNAs (lncRNAs) are transcribed RNA molecules that can interact with DNA or RNA, transcription factors, histones and other chromatin modifying proteins, affecting the expression level of a broad spectrum of genes. Because of their tissue-specific expression characteristics, lncRNAs hold strong promise as novel biomarkers and therapeutic targets for diseases. High-density whole-genome microarray analysis in ameloblastomas, an aggressive odontogenic tumor, and in adenomatoid odontogenic tumor (AOT), an indolent one, showed a high frequency of copy number alteration at chromosome band 14q32.33, which encompasses the lncRNA gene KIAA0125. To understand molecular mechanisms associated with their biological behavior and considering the copy-number gains observed at 14q32.33, we aimed to investigate the expression levels of the lncRNA KIAA0125 in ameloblastomas and in AOTs. The University Ethics Committee approved this study and patients signed informed consent. Thirteen frozen samples were included: five solid/multicystic ameloblastomas, four AOT, and four dental follicles. qPCR reactions were performed in triplicates and the relative changes in gene expression were obtained using the 2^-ΔΔCt formula. The reference gene HPRT1 was used for normalization and the human keratinocyte HaCat cell line was used as calibrator. Differences in the relative changes in gene expression between two groups of samples were assessed and the significance level was set at 0.05. All samples expressed more KIAA0125 than the calibrator. The ameloblastoma group showed higher expression levels of KIAA0125 when compared to dental follicles (p=0.042), while there was no difference between the expression in ameloblastomas and AOT (p>0.05). The expression levels of KIAA0125 in AOT were not different from that of the dental follicle. Our findings suggest that lncRNA KIAA0125 is likely involved in ameloblastoma pathobiology. LncRNAs hold strong promise as therapeutic targets, and experimental validation of this lncRNA functions may lead to tailored therapies targeting KIAA0125 in extensive and recurrent ameloblastomas.
local.identifier.orcidhttps://orcid.org/0000-0001-7820-4749
local.identifier.orcidhttps://orcid.org/0000-0002-4212-1172
local.identifier.orcidhttps://orcid.org/0000-0002-6005-783X
local.identifier.orcidhttps://orcid.org/0000-0001-6325-4145
local.identifier.orcidhttps://orcid.org/0000-0002-0732-7183
local.identifier.orcidhttps://orcid.org/0000-0002-7427-0578
local.identifier.orcidhttps://orcid.org/0000-0001-8770-8009
local.identifier.orcidhttps://orcid.org/0000-0003-1580-4995
local.publisher.countryBrasil
local.publisher.departmentFAO - DEPARTAMENTO DE CLÍNICA
local.publisher.departmentICB - DEPARTAMENTO DE PATOLOGIA
local.publisher.initialsUFMG
local.url.externahttps://aacrjournals.org/cancerres/article/79/13_Supplement/1811/542299/Abstract-1811-The-long-noncoding-RNA-KIAA0125-is

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