Label-free impedimetric immunosensors for detection of snake venoms using polyaniline as a transducer substrate

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dc.creatorRicardo Adriano Dorledo de Faria
dc.creatorPedro Henrique Cabral de Souza
dc.creatorVanessa de Freitas Cunha Lins
dc.creatorTulio Matencio
dc.creatorLuiz Guilherme Dias Heneine
dc.date.accessioned2023-08-07T13:09:19Z
dc.date.accessioned2025-09-09T00:36:09Z
dc.date.available2023-08-07T13:09:19Z
dc.date.issued2020
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.description.sponsorshipOutra Agência
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.26717/BJSTR.2020.25.004190
dc.identifier.issn2574-1241
dc.identifier.urihttps://hdl.handle.net/1843/57516
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofBiomedical Journal of Scientific & Technical Research
dc.rightsAcesso Aberto
dc.subjectEletroquímica
dc.subjectBiossensores
dc.subjectEspectroscopia de impedância
dc.subjectCobra venenosa - Veneno
dc.subjectEletrodeposição
dc.subjectPolímeros condutores
dc.subjectReações antígeno-anticorpo
dc.subject.otherImmunosensor
dc.subject.otherElectrochemical impedance spectroscopy
dc.subject.otherPolyaniline
dc.subject.otherSnake venom
dc.titleLabel-free impedimetric immunosensors for detection of snake venoms using polyaniline as a transducer substrate
dc.typeArtigo de periódico
local.citation.epage19091
local.citation.issue3
local.citation.spage19083
local.citation.volume25
local.description.resumoTwo label-free impedimetric immunosensors were developed to be employed in the specific diagnosis of snakebite. To produce the devices, a conductive substrate was obtained by electrodeposition of Polyaniline on the surface of a Crofer 22 APU steel. The polymer-based substrates were functionalized either with an affinity purified antibody specific for venom of the Bothrops genus or for the Crotalus genus. The performance of the as-prepared sensors was evaluated by exposing them to different concentrations of the venoms from Bothrops, Crotalus and Micrurus snakes. The capacity to recognize the specific analytes was measure by Electrochemical Impedance Spectroscopy within a frequency range from 10,000 Hz to 0.01 Hz. The increase of the immunosensors’ charge transfer resistance was correlated to the increase in the concentration of the homologous venoms pool with a detection limit of 0.1 μg. m L-¹. Heterologous venoms were not detected at any tested concentration, proving the expected high selectivity. Furthermore, the results showed the reusability of the sensor, as it retained the capability of detecting the specific analytes after many regeneration cycles. The use of immunosensors to detect snake venom shows important promise as an aid to snakebite treatment.
local.identifier.orcidhttps://orcid.org/0000-0002-7025-7042
local.identifier.orcidhttps://orcid.org/0000-0002-2071-9309
local.identifier.orcidhttps://orcid.org/0000-0002-6357-9553
local.identifier.orcidhttps://orcid.org/0000-0002-5660-8125
local.identifier.orcidhttps://orcid.org/0000-0003-0707-2691
local.publisher.countryBrasil
local.publisher.departmentENG - DEPARTAMENTO DE ENGENHARIA QUÍMICA
local.publisher.departmentICX - DEPARTAMENTO DE QUÍMICA
local.publisher.initialsUFMG
local.url.externahttps://biomedres.us/fulltexts/BJSTR.MS.ID.004190.php

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