Effect of different extraction methods on anthocyanin content in hibiscus sabdariffa L. and their antiplatelet and vasorelaxant properties

Página do item simplificado
dc.creatorDenise Rubinho Dos Santos Martins
dc.creatorCaroline Honaiser Lescano
dc.creatorAlberto Fernando Oliveira Justo
dc.creatorJulia Modesto Vicente
dc.creatorSérgio Henrique Sousa Santos
dc.creatorCharles Martins Aguilar
dc.creatorAlexandre Borges
dc.creatorIvan Pires de Oliveira
dc.creatorEliana Janet Sanjinez-Argandoña
dc.date.accessioned2024-09-24T12:10:12Z
dc.date.accessioned2025-09-09T00:23:42Z
dc.date.available2024-09-24T12:10:12Z
dc.date.issued2023
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipFAPESP - Fundação de Amparo à Pesquisa do Estado de São Paulo
dc.description.sponsorshipOutra Agência
dc.identifier.doihttps://doi.org/10.1007/s11130-023-01067-5
dc.identifier.issn09219668
dc.identifier.urihttps://hdl.handle.net/1843/76838
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofPlant Foods for Human Nutrition
dc.rightsAcesso Restrito
dc.subjectNucleotídeos cíclicos
dc.subjectComputadores moleculares
dc.subjectPlaquetas (Sangue)
dc.subjectHibisco
dc.subject.otherNucleotídeos cíclicos
dc.subject.otherComputadores moleculares
dc.titleEffect of different extraction methods on anthocyanin content in hibiscus sabdariffa L. and their antiplatelet and vasorelaxant properties
dc.typeArtigo de periódico
local.citation.epage350
local.citation.issue2
local.citation.spage342
local.citation.volume78
local.description.resumoHibiscus sabdariffa L. is a worldwide component for tea and beverages, being a natural source of anthocyanins, which are associated with cardiovascular activities. To investigate this relationship, we explored different methods of aqueous extraction on the anthocyanin content and antioxidant activity of H. sabdariffa L. calyx extract (HSCE). Pharmacological effects via platelet aggregation, calcium mobilization, cyclic nucleotide levels, vasodilator-stimulated phosphoprotein Ser157 and Ser239, and on the vasomotor response of aortic rings isolated from mice are studied herewith. We found that the application of ultrasonic turbolization, 20 min, combined with acidified water was significantly more effective in the extraction process, providing extracts with the highest levels of anthocyanins (8.73 and 9.63 mg/100 g) and higher antioxidant activity (6.66 and 6.78 μM trolox/g of sample). HSCE significantly inhibited (100-1000 μg/mL) arachidonic acid-induced platelet aggregation, reduced calcium mobilization, and increased cAMP and cGMP levels with VASPSer157 and VASPSer239 phosphorylation. Vasorelaxation reduction was confirmed by the aortic rings and endothelium assays treated with nitric oxide synthase inhibitors, soluble guanylyl cyclase (sGC) oxidizing agent, or Ca2+-activated K+ channel inhibitor. The increasing of cGMP levels could be understood considering the sGC stimulation by HSCE compounds in the specific stimulus domain, which allows an understanding of the observed antiplatelet and vasorelaxant properties of H. sabdariffa L. calyx extract.
local.publisher.countryBrasil
local.publisher.departmentICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS
local.publisher.initialsUFMG
local.url.externahttps://pubmed.ncbi.nlm.nih.gov/37120677/

Arquivos

Licença do pacote

Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
License.txt
Tamanho:
1.99 KB
Formato:
Plain Text
Descrição:
Baixar

Coleções

Artigo de Periódico

Logo UFMG