MicroRNAs 145 and 148a are upregulated during congenital Zika Virus Infection

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dc.creatorFernanda L. Castro
dc.creatorAlessandro L. Gonçalves
dc.creatorDaniela P. Cunha
dc.creatorMaria Elisabeth Moreira
dc.creatorZilton Farias Meira de Vasconcelos
dc.creatorLeila Chimeli
dc.creatorAdriana Melo
dc.creatorAmilcar Tanuri
dc.creatorDouglas Nixon
dc.creatorMarcelo Ribeiro Alves
dc.creatorRenato Santana de Aguiar
dc.creatorVictor Geddes
dc.creatorFábio Luís Lima Monteiro
dc.creatorRaphael M. D. T. Gonçalves
dc.creatorLoraine Campanati Araujo de Andrade
dc.creatorPaula Pezzuto
dc.creatorDominic Paquin Proulx
dc.creatorBruno Luiz Fonseca Schamber Reis
dc.creatorGirlene S. Azevedo
dc.date.accessioned2023-07-15T00:14:52Z
dc.date.accessioned2025-09-09T01:33:09Z
dc.date.available2023-07-15T00:14:52Z
dc.date.issued2019
dc.identifier.doihttps://doi.org/10.1177/1759091419850983
dc.identifier.issn1759-0914
dc.identifier.urihttps://hdl.handle.net/1843/56350
dc.languagepor
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofASN Neuro
dc.rightsAcesso Aberto
dc.subjectRetinoblastoma
dc.subjectVírus da Zika
dc.subjectMicrocefalia
dc.subject.othermiRNAs
dc.subject.otherMicrocephaly
dc.subject.otherNeuroblastoma
dc.subject.otherPostmortem brain autopsies
dc.subject.otherZika
dc.titleMicroRNAs 145 and 148a are upregulated during congenital Zika Virus Infection
dc.typeArtigo de periódico
local.citation.epage14
local.citation.spage1
local.citation.volume11
local.description.resumoAbstract Zika virus (ZIKV) is an arthropod-borne virus (arbovirus) member of the Flaviviridae family, which has been associated with the development of the congenital Zika syndrome (CZS). RNA viruses, such as flaviviruses, have been reported to exert a profound impact on host microRNAs (miRNAs). Cellular miRNAs modulated by ZIKV may help identify cellular pathways of relevance to pathogenesis. Here, we screened 754 human cellular miRNAs modulated by ZIKV infection (Brazilian PE strain) in a neuroblastoma cell line. Seven miRNAs (miR-99a*, miR-126*, miR-190b, miR-361-3p, miR-522-3p, miR-299-5p, and miR1267) were downregulated during ZIKV infection, while miR-145 was upregulated. Furthermore, 11 miRNAs were exclusively expressed in ZIKV-infected (miR-148a, miR-342-5p, miR-598, and miR-708-3p) or mock cells (miR-208, miR-329, miR432-5p, miR-488, miR-518b, miR-520g, and miR-767-5p). Furthermore, in silico analysis indicated that some central nervous system, cellular migration, and adhesion function-related biological processes were overrepresented in the list of target genes of the miRNAs regulated in ZIKV-infected cells, especially for miR-145 and miR-148a. The induction of miR-145 and miR-148a was confirmed in postmortem brain samples from stillborn with severe CZS. Finally, we determined the expression regulation of microcephaly related genes through RNA interference pathway caused by ZIKV directly on neuron cells.
local.identifier.orcidhttps://orcid.org/0000-0001-9381-385X
local.identifier.orcidhttps://orcid.org/0000-0002-2193-2224
local.identifier.orcidhttps://orcid.org/0000-0003-0570-750X
local.identifier.orcidhttps://orcid.org/0000-0002-2801-1786
local.identifier.orcidhttps://orcid.org/0000-0002-8663-3364
local.identifier.orcidhttps://orcid.org/0000-0001-5180-3717
local.identifier.orcidhttps://orcid.org/0000-0002-0723-3873
local.identifier.orcidhttps://orcid.org/0000-0002-9107-6865
local.identifier.orcidhttps://orcid.org/0000-0001-9172-1951
local.identifier.orcidhttps://orcid.org/0000-0001-9570-8244
local.identifier.orcidhttps://orcid.org/0000-0003-1407-3414
local.identifier.orcidhttps://orcid.org/0000-0002-7640-1463
local.publisher.countryBrasil
local.publisher.departmentICB - INSTITUTO DE CIÊNCIAS BIOLOGICAS
local.publisher.initialsUFMG
local.url.externahttps://journals.sagepub.com/doi/10.1177/1759091419850983

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