Testosterone improves the osteogenic potential of a composite in vitro and in vivo

Página do item simplificado
dc.creatorKelen Jorge Rodrigues da Costa Nunes
dc.creatorAlfonso Gala García
dc.creatorJoel Jose Passos
dc.creatorVagner Rodrigues Santos
dc.creatorRubén Dario Sinisterra Millán
dc.creatorCélia Regina Moreira Lanza
dc.creatorMaría Esperanza Cortés Segura
dc.date.accessioned2024-01-26T17:12:15Z
dc.date.accessioned2025-09-08T22:57:16Z
dc.date.available2024-01-26T17:12:15Z
dc.date.issued2019
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipINCT – Instituto nacional de ciência e tecnologia (Antigo Instituto do Milênio)
dc.identifier.doihttps://doi.org/10.1007/s00441-018-2970-3
dc.identifier.issn1432-0878
dc.identifier.urihttps://hdl.handle.net/1843/63427
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofCell and Tissue Research
dc.rightsAcesso Restrito
dc.subjectTestosterona
dc.subjectFosfato de cálcio
dc.subjectBiomineralização
dc.subjectOssos - Crescimento
dc.subjectOsseointegração
dc.subject.otherTestosterone
dc.subject.otherBone formation
dc.subject.otherCalcium phosphate
dc.subject.otherCeramic composite
dc.subject.otherMineralization
dc.titleTestosterone improves the osteogenic potential of a composite in vitro and in vivo
dc.typeArtigo de periódico
local.citation.epage231
local.citation.spage221
local.citation.volume376
local.description.resumoTestosterone (T) has been suggested as a promising agent in the bone osteointegration when incorporated in a bioceramic/polymer combination for the local application. The objective of this study was to evaluate the activity of a testosterone composite of poly (lactic-co-glycolic acid) (PLGA), polycaprolactone (PCL), and biphasic calcium phosphate (BCP) as a strategy for enhancing its osteogenic effect and to evaluate tissue response to the composite implantation. PLGA/PCL/BCP/T and PLGA/PCL/BCP composites were prepared and characterized using thermal analysis. Composite morphology and surface characteristics were assessed by SEM and EDS. The evaluations of in vitro effects of testosterone composite on osteoblasts viability, alkaline phosphatase activity, collagen production, osteocalcin concentration, quantification of mineralization, and nitric oxide concentration, after 7, 14, and 21 days. Testosterone was successfully incorporated and composites showed a homogeneously distributed porous structure. The PLGA/PCL/BCP/T composite had a stimulatory effect on osteoblastic activity on the parameters evaluated, except to nitric oxide production. After 60 days, the PLGA/PCL/BCP/T composite showed no chronic inflammatory infiltrate, whereas the PLGA/PCL/BCP composite showed mild chronic inflammatory infiltrate. Angiogenesis, cellular adsorption, and fibrous deposit were observed on the surfaces of implanted composites. The composites in combination with testosterone can be exploited to investigate the use of this scaffold for bone integration.
local.identifier.orcidhttps://orcid.org/0000-0002-3934-1094
local.identifier.orcidhttps://orcid.org/0000-0002-5370-4867
local.identifier.orcidhttps://orcid.org/0000-0001-7656-1849
local.identifier.orcidhttps://orcid.org/0000-0001-5893-2597
local.identifier.orcidhttps://orcid.org/0000-0002-0560-8491
local.publisher.countryBrasil
local.publisher.departmentFAO - DEPARTAMENTO DE CLÍNICA
local.publisher.departmentFAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORA
local.publisher.departmentICX - DEPARTAMENTO DE QUÍMICA
local.publisher.initialsUFMG
local.url.externahttps://link.springer.com/article/10.1007/s00441-018-2970-3

Arquivos

Licença do pacote

Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
License.txt
Tamanho:
1.99 KB
Formato:
Plain Text
Descrição:
Baixar

Coleções

Artigo de Periódico

Logo UFMG