Staphylococcus aureus-Cure-Associated Antigens Elicit Type 3 Immune Memory T Cells

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dc.creatorKamila R. Santos
dc.creatorAngélica Rosa Faria
dc.creatorHélida Monteiro de Andrade
dc.creatorMônica M. O. P. Cerqueira
dc.creatorMagnus Gidlund
dc.creatorHiro Goto
dc.creatorAlice Maria M. P. Della Libera
dc.creatorFernando N. Souza
dc.creatorEduardo M. Ramos-Sanchez
dc.creatorCamila F. Batista
dc.creatorLuiza C. Reis
dc.creatorWesley L. Fotoran
dc.creatorMarcos B. Heinemann
dc.creatorAdriano F. Cunha
dc.creatorMussya C. Rocha
dc.date.accessioned2023-10-30T21:05:41Z
dc.date.accessioned2025-09-09T01:29:28Z
dc.date.available2023-10-30T21:05:41Z
dc.date.issued2022-10-02
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.3390/antibiotics11121831
dc.identifier.issn2079-6382
dc.identifier.urihttps://hdl.handle.net/1843/60283
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofAntibiotics
dc.rightsAcesso Aberto
dc.subjectVacinação
dc.subjectCélulas T.
dc.subjectInfecções
dc.subjectLaticínios
dc.subject.otherVaccine
dc.subject.otherT cell response
dc.subject.otherIL-17A
dc.subject.otherIntramammary infection
dc.subject.otherStaphylococcus aureus
dc.subject.otherMastitis
dc.subject.otherRecombinant antigens
dc.subject.otherDairy cow
dc.titleStaphylococcus aureus-Cure-Associated Antigens Elicit Type 3 Immune Memory T Cells
dc.typeArtigo de periódico
local.citation.epage12
local.citation.issue12
local.citation.spage1831
local.citation.volume11
local.description.resumoBackground: Staphylococcus aureus is one of the most frequently major mastitis pathogens that cause clinical and subclinical mastitis worldwide. Current antimicrobial treatments are usually ineffective, and the commercially available vaccines lack proven effectiveness. The immunological response elicited by the recombinant S. aureus-cure-associated proteins phosphoglycerate kinase (PGK), enolase (ENO), and elongation factor-G (EF-G) in combination with the granulocyte-macrophage colony-stimulating factor (GM-CSF) DNA vaccination was studied in this work. Methods: Here, twenty-three C57BL/6 mice were divided into four groups and vaccinated with: G1: none (control); G2: GM-CSF DNA plasmid DNA vaccine; G3: the combination of EF-G+ENO+PGK; and G4: the combinations of EF-G+ENO+PGK proteins plus GM-CSF plasmid DNA vaccine. After 44 days, spleen cells were collected for immunophenotyping and lymphocyte proliferation evaluation by flow cytometry upon S. aureus stimulus. Results: Immunization with the three S. aureus recombinant proteins alone resulted in a higher percentage of IL-17A+ cells among CD8+ T central memory cells, as well as the highest intensity of IL-17A production by overall lymphocytes indicating that the contribution of the combined lymphocyte populations is crucial to sustaining a type 3 cell immunity environment. Conclusion: The immunization with three S. aureus-cure-associated recombinant proteins triggered type 3 immunity, which is a highly interesting path to pursue an effective bovine S. aureus mastitis vaccine.
local.identifier.orcidhttps://orcid.org/0000-0001-9476-202X
local.identifier.orcidhttps://orcid.org/0000-0002-5380-0939
local.identifier.orcidhttps://orcid.org/0000-0001-8700-5469
local.identifier.orcidhttps://orcid.org/0000-0001-5412-4066
local.identifier.orcidhttps://orcid.org/0000-0001-8203-8384
local.identifier.orcidhttps://orcid.org/0000-0001-6444-9788
local.identifier.orcidhttps://orcid.org/0000-0001-9118-8464
local.identifier.orcidhttps://orcid.org/0000-0002-7246-2648
local.identifier.orcidhttps://orcid.org/0000-0003-2047-1136
local.identifier.orcidhttps://orcid.org/0000-0001-7048-562X
local.identifier.orcidhttps://orcid.org/0000-0001-5767-5920
local.publisher.countryBrasil
local.publisher.departmentFAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICAS
local.publisher.departmentFARMACIA - FACULDADE DE FARMACIA
local.publisher.departmentICB - DEPARTAMENTO DE PARASITOLOGIA
local.publisher.initialsUFMG
local.url.externahttps://www.mdpi.com/2079-6382/11/12/1831

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