Identificação espécie-específica e de pureza em produtos de origem bubalina por meio de técnicas moleculares

Abstract

The research conducted at the UFMG genetics laboratory seeks the implementation of product authenticity test originating from buffalo (Bubalus bubalis) and addition detection not-declared of bovine material based on molecular biology. For the performance of this study, it was used the chi-square statistical test comparing different forms of DNA extraction from food products, in addition to testing the effectiveness of PCR-RFLP technique (polymerase chain reaction - restriction fragment length polymorphism), which presents very high specificity and good sensibility. This technique can also be extended for other species and it is viable for application in the laboratorial routine and it is indicated for inspection of the authenticity of animal foods origin.