Synthesis and characterization of a molecularly imprinted polymer (MIP) for solid-phase extraction of the antidiabetic gliclazide from human plasma

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dc.creatorIngrid Vasconcelos
dc.creatorPedro Henrique Reis da Silva
dc.creatorDerick Rodrigues Davila Dias
dc.creatorMaria Betânia de Freitas Marques
dc.creatorWagner da Nova Mussel
dc.creatorTércio Assunção Pedrosa
dc.creatorMaria Elisa Scarpelli Ribeiro e Silva
dc.creatorRoberto Fernando de Souza Freitas
dc.creatorRicardo Geraldo de Sousa
dc.creatorChristian Fernandes
dc.date.accessioned2022-05-02T20:11:44Z
dc.date.accessioned2025-09-08T23:57:48Z
dc.date.available2022-05-02T20:11:44Z
dc.date.issued2020-11
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.description.sponsorshipOutra Agência
dc.format.mimetypepdf
dc.identifier.doi10.1016/j.msec.2020.111191
dc.identifier.issn0928-4931
dc.identifier.urihttps://hdl.handle.net/1843/41299
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofMaterials Science and Engineering: C
dc.rightsAcesso Aberto
dc.subjectDiabetes mellitus
dc.subjectGliclazida
dc.subject.otherDiabetes mellitus
dc.subject.otherSulphonylureas
dc.subject.otherMolecularly imprinted solid-phase extraction
dc.subject.otherSample preparation
dc.titleSynthesis and characterization of a molecularly imprinted polymer (MIP) for solid-phase extraction of the antidiabetic gliclazide from human plasma
dc.typeArtigo de periódico
local.citation.epage3
local.citation.spage1
local.citation.volume116
local.description.resumoGliclazide is a sulfonylurea frequently prescribed for the management of type 2 diabetes mellitus in elderly patients and for patients with chronic renal or hepatic diseases. Even though it is considered a safer alternative, the drug can provoke side effects in some patients, especially hypoglycemia, due to the high interindividual variability. Therefore, the quantification of gliclazide in biological samples is usually recommended in order to assure efficacy and safety of the pharmacotherapy. However, due to the complexity of biological matrices, therapeutic monitoring can be very challenging, especially in the sample preparation step. For that reason, the synthesis and characterization of a novel and selective molecularly imprinted polymer (MIP) was proposed to be employed as sorbent for the extraction of gliclazide from human plasma samples by a molecularly imprinted solid-phase extraction (MISPE) procedure. Synthesis conditions were optimized (monomer, crosslinker and porogen) and the polymer was characterized for its morphological, physicochemical and stability properties. The influence of drug concentration, solvent composition and pH on the coefficient of distribution (Kd) and imprinting factor (IF) were studied, as well as repeatability between batches and selectivity. A bioanalytical method was developed applying the developed MIP as sorbent in solid phase extraction and liquid chromatography using a Poroshell 120 C18 (100 × 4.6 mm, 4 μm) column, acetonitrile and 10 mM potassium phosphate buffer pH 3.0 (50:50) at a flow-rate of 1.2 mL/min as mobile phase, temperature of 30 °C, injection volume of 40 μL and detection at 230 nm. The best reaction yield, extraction capacity, and selectivity was obtained using 2-hydroxyethyl methacrylate (2-HEMA), ethyleneglycol dimethacrylate (EGDMA) and acetonitrile. The optimized MIP showed coefficient of distribution (Kd) of 59.85 μg/g, imprinting factor (IF) of 1.60, and selectivity for gliclazide and other sulfonylureas compared to possible concurrent drugs. The developed method by MISPE-HPLC-UV showed to be appropriate to determine gliclazide in human plasma samples.
local.publisher.countryBrasil
local.publisher.departmentFAR - DEPARTAMENTO DE ALIMENTOS
local.publisher.departmentFAR - DEPARTAMENTO DE PRODUTOS FARMACÊUTICOS
local.publisher.initialsUFMG
local.url.externahttps://www.sciencedirect.com/science/article/pii/S0928493120316891

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