Liquid chromatography-tandem mass spectrometry bioanalytical method for the determination of kavain in mice plasma: application to a pharmacokinetic study

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dc.creatorJuliana Veloso Ferreira
dc.creatorAlysson Vinícius Braga
dc.creatorRenes de Resende Machado
dc.creatorDeborah Michel
dc.creatorGerson Antônio Pianetti
dc.creatorAnas El-Aneed
dc.creatorIsabela Costa César
dc.date.accessioned2026-01-22T18:40:59Z
dc.date.issued2020-01-15
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.description.sponsorshipOutra Agência
dc.identifier.doihttps://doi.org/10.1016/j.jchromb.2019.121939
dc.identifier.issn1873-376X
dc.identifier.urihttps://hdl.handle.net/1843/1474
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofJournal of Chromatography B
dc.rightsAcesso restrito
dc.subjectFarmacocinética
dc.subjectCromatografia líquida
dc.subjectEspectrometria de massas
dc.subject.otherLC-MS/MS
dc.subject.otherValidação do método
dc.subject.otherKavaina
dc.subject.otherKava-kava
dc.subject.otherEstudo farmacocinético
dc.titleLiquid chromatography-tandem mass spectrometry bioanalytical method for the determination of kavain in mice plasma: application to a pharmacokinetic study
dc.typeArtigo de periódico
local.citation.epage8
local.citation.spage1
local.citation.volume1137
local.description.resumoA simple and fast bioanalytical method for the quantification of kavain in mice plasma was developed using liquid chromatography (LC)-tandem mass spectrometry (MS/MS). A full method validation was performed, according to regulatory guidelines, employing isotopically labeled kavain as the internal standard (racemic-kavain-d3). For the quantification, [M+H]+ was formed using an electrospray ionization (ESI) source in the positive ion mode and multiple reaction monitoring (MRM) was employed using a quadrupole-linear ion trap (4000 QTRAP®) instrument. The monitored MRM transitions were 231.0 → 115.1 and 231.0 → 152.8 for kavain; and 234.2 → 199.2 for the internal standard. A linear response was obtained at the concentration range of 10 to 200 ng/mL with intra- and inter-day variations within the acceptable criteria for all quality control samples. After validation, the method was successfully applied for the quantification of kavain in mice plasma after oral administration of the kavain standard and Kava-kava extract. The plasma concentration over time results were applied for a pharmacokinetics study. The obtained pharmacokinetic parameters indicated a considerably higher bioavailability for kavain when Kava-kava extract was administered due to a pharmacokinetic synergism between the analyte and the other compounds present in the extract.
local.publisher.countryBrasil
local.publisher.departmentFAR - DEPARTAMENTO DE PRODUTOS FARMACÊUTICOS
local.publisher.initialsUFMG
local.subject.cnpqCIENCIAS DA SAUDE
local.url.externahttps://www.sciencedirect.com/science/article/pii/S157002321931428X?via%3Dihub

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