Total combining power: technique for the evaluation of the quality control process of clostridiosis vaccines

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dc.creatorIgor Viana Brandi
dc.creatorEliane Macedo Sobrinho Santos
dc.creatorBruna Mara Aparecida de Carvalho Mesquita
dc.creatorCarla Adriana Ferreira Durães
dc.creatorPaula Karoline Soares Farias
dc.creatorRoger Shoji Sari
dc.creatorAlex Sander Rodrigues Cangussu
dc.creatorAdalberto Pessoa
dc.date.accessioned2022-06-23T11:14:04Z
dc.date.accessioned2025-09-09T00:34:51Z
dc.date.available2022-06-23T11:14:04Z
dc.date.issued2016
dc.description.sponsorshipOutra Agência
dc.identifier.doihttps://doi.org/10.1016/j.mimet.2016.08.023
dc.identifier.issn0167-7012
dc.identifier.urihttps://hdl.handle.net/1843/42608
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofJournal of Microbiological Methods
dc.rightsAcesso Aberto
dc.subjectVacina veterinária
dc.subjectClostridioses
dc.subjectToxinas bacterianas
dc.titleTotal combining power: technique for the evaluation of the quality control process of clostridiosis vaccines
dc.title.alternativePoder total de combinação: Técnica para avaliação do processo de controle de qualidade de vacinas contra clostridiose
dc.typeArtigo de periódico
local.citation.epage168
local.citation.spage164
local.citation.volume130
local.description.resumoAn efficient technique for evaluation of the quality control of vaccines against clostridiosis is described in this study. This technique is capable of quantifying the toxoid of the bacterium Clostridium perfringens Type D, which is commonly found within these vaccines. The described method is performed in vivo to quantify the toxoid, replacing the current predominant approaches that use the titration of toxins before the inactivation process. This method is based on the partial neutralization of a determined dose of antitoxin by testing different doses of the toxoid. In order to guarantee its reliability, it is essential for the technique to be validated. Thus, the technique was tested using the following validation parameters: specificity and selectivity, detection limit, linear correlation, precision and robustness, in agreement with the requirements of regulatory agencies and international committees from around the world. The method was found to be specific, selective, robust, precise, and linear inside a specific concentration range. Therefore, it could be applied to the quality control of clostridiosis vaccines with satisfactory results.
local.identifier.orcidhttps://orcid.org/0000-0001-6714-7996
local.identifier.orcidhttps://orcid.org/ 0000-0001-8980-8599
local.publisher.countryBrasil
local.publisher.departmentICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS
local.publisher.initialsUFMG
local.url.externahttps://www.sciencedirect.com/science/article/pii/S0167701216302330

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