A new method for studying cryptococcosis in a murine model using 99mTc-Cryptococcus gattii

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dc.creatorMarliete Carvalho Costa
dc.creatorLays Murta Mata
dc.creatorNoelly de Queiroz Ribeiro
dc.creatorAnderson Philip Nonato Santos
dc.creatorLorena Vivien Neves Oliveira
dc.creatorRaquel Virgínia Rocha Vilela
dc.creatorValbert Nascimento Cardoso
dc.creatorSimone Odília Antunes Fernandes
dc.creatorDaniel Assis Santos
dc.date.accessioned2022-05-03T01:20:43Z
dc.date.accessioned2025-09-08T22:51:29Z
dc.date.available2022-05-03T01:20:43Z
dc.date.issued2018-06
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.format.mimetypepdf
dc.identifier.doi10.1093/mmy/myx060
dc.identifier.issn1369-3786
dc.identifier.urihttps://hdl.handle.net/1843/41308
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofMedical Mycology
dc.rightsAcesso Aberto
dc.subjectMicose
dc.subjectCryptococcus gattii
dc.subject.otherAnimal model
dc.subject.otherFungal dissemination
dc.subject.otherRadiolabeling
dc.subject.otherCryptococcus gattii
dc.subject.otherTechnetium-99m
dc.titleA new method for studying cryptococcosis in a murine model using 99mTc-Cryptococcus gattii
dc.typeArtigo de periódico
local.citation.epage484
local.citation.issue4
local.citation.spage479
local.citation.volume56
local.description.resumoCryptococcus gattii is one of the etiologic agents of cryptococcosis, a systemic mycosis that occurs in healthy and immunosuppressed humans and animals worldwide. Primary pulmonary infection caused by C. gattii is usually followed by fungal dissemination to the central nervous system, resulting in high mortality rates. In this context, animal models of cryptococcosis are useful in the study of fungal pathogenesis and host response against the pathogen, and for testing novel therapeutic options. The most frequently applied method to study fungal dissemination from the lungs to other organs is by culturing tissues, which is not accurate for the detection and quantification of fungal load at early stages of the infection. To overcome this problem, the purpose of this study was to develop a new method for the quantification of Cryptococcus dissemination. One C. gattii strain was efficiently radiolabeled with technetium-99m (99mTc), without affecting viability of the cells. Further, the 99mTc-C. gattii (111 MBq) strain was used to infect mice by intratracheal and intravenous route for biodistribution studies. 99mTc-C. gattii was successfully used in detection of the yeast in the brain of mice 6 hours postinoculation, while the detection using colony forming units was possible only 24 hours postinfection. Our results provided an alternative method that could be applied in further investigations regarding the efficacy of antifungals, fungal virulence, and host-pathogen interactions.
local.publisher.countryBrasil
local.publisher.departmentFAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICAS
local.publisher.initialsUFMG
local.url.externahttps://academic.oup.com/mmy/article-lookup/doi/10.1093/mmy/myx060

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