Chorioamnionitis Induces a Specific Signature of Placental ABC Transporters Associated with an Increase of miR-331-5p in the Human Preterm Placenta

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dc.creatorGuinever Eustaquio do Imperio
dc.creatorTania Maria Ortiga Carvalho
dc.creatorStephen Giles Matthews
dc.creatorEnrrico Bloise
dc.creatorMohsen Javam
dc.creatorPhetcharawan Lye
dc.creatorAndrea Constantinof
dc.creatorCaroline Dunk
dc.creatorFernando Marcos Dos Reis
dc.creatorStephen James Lye
dc.creatorWilliam Gibb
dc.date.accessioned2023-10-05T22:26:33Z
dc.date.accessioned2025-09-08T23:28:03Z
dc.date.available2023-10-05T22:26:33Z
dc.date.issued2018
dc.identifier.doihttps://doi.org/10.1159/000487100
dc.identifier.issn1015-8987
dc.identifier.urihttps://hdl.handle.net/1843/59206
dc.languagepor
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofCellular Physiology and Biochemistry
dc.rightsAcesso Aberto
dc.subjectPlacenta
dc.subjectCâncer de mama
dc.subjectGlicoproteinas
dc.subject.otherPlacenta
dc.subject.otherChorioamnionitis
dc.subject.otherPreterm delivery
dc.subject.otherABC transporters
dc.subject.otherP-glycoprotein (P-gp)
dc.subject.otherBreast cancer resistance protein (BCRP)
dc.titleChorioamnionitis Induces a Specific Signature of Placental ABC Transporters Associated with an Increase of miR-331-5p in the Human Preterm Placenta
dc.typeArtigo de periódico
local.citation.epage604
local.citation.issue2
local.citation.spage591
local.citation.volume45
local.description.resumoBackground/Aims: The ATP-binding cassette (ABC) transporters mediate drug biodisposition and immunological responses in the placental barrier. In vitro infective challenges alter expression of specific placental ABC transporters. We hypothesized that chorioamnionitis induces a distinct pattern of ABC transporter expression. Methods: Gene expression of 50 ABC transporters was assessed using TaqMan® Human ABC Transporter Array, in preterm human placentas without (PTD; n=6) or with histological chorioamnionitis (PTDC; n=6). Validation was performed using qPCR, immunohistochemistry and Western blot. MicroRNAs known to regulate P-glycoprotein (P-gp) were examined by qPCR. Results: Up-regulation of ABCB9, ABCC2 and ABCF2 mRNA was detected in chorioamnionitis (p<0.05), whereas placental ABCB1 (P-gp; p=0.051) and ABCG2 (breast cancer resistance protein-BCRP) mRNA levels (p=0.055) approached near significant up-regulation. In most cases, the magnitude of the effect significantly correlated to the severity of inflammation. Upon validation, increased placental ABCB1 and ABCG2 mRNA levels (p<0.05) were observed. At the level of immunohistochemistry, while BCRP was increased (p<0.05), P-gp staining intensity was significantly decreased (p<0.05) in PTDC. miR-331-5p, involved in P-gp suppression, was upregulated in PTDC (p<0.01) and correlated to the grade of chorioamnionitis (p<0.01). Conclusions: Alterations in the expression of ABC transporters will likely lead to modified transport of clinically relevant compounds at the inflamed placenta. A better understanding of the potential role of these transporters in the events surrounding PTD may also enable new strategies to be developed for prevention and treatment of PTD.
local.identifier.orcidhttps://orcid.org/0000-0002-5744-6623
local.identifier.orcidhttps://orcid.org/0000-0003-0275-8686
local.identifier.orcidhttps://orcid.org/0000-0001-5063-1773
local.publisher.countryBrasil
local.publisher.departmentICB - DEPARTAMENTO DE MORFOLOGIA
local.publisher.departmentMED - DEPARTAMENTO DE GINECOLOGIA OBSTETRÍCIA
local.publisher.initialsUFMG
local.url.externahttps://karger.com/cpb/article/45/2/591/74452/Chorioamnionitis-Induces-a-Specific-Signature-of

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