Leader gene of corynebacterium pseudotuberculosis may be useful in vaccines against caseous lymphadenitis of goats: a bioinformatics approach

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dc.creatorEliane Macedosobrinho Santos
dc.creatorAnna Christina de Almeida
dc.creatorHércules Otacílio Santos
dc.creatorAlexsander Rodrigues Cangussu
dc.creatorDeborah Aires Almeida
dc.creatorKattyanne Souza Costa
dc.date.accessioned2022-06-09T11:06:03Z
dc.date.accessioned2025-09-09T00:40:34Z
dc.date.available2022-06-09T11:06:03Z
dc.date.issued2018
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.description.sponsorshipOutra Agência
dc.identifier.doihttp://doi.org/10.1292/jvms.16-0581
dc.identifier.issn1347-7439
dc.identifier.urihttps://hdl.handle.net/1843/42376
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofJournal of Veterinary Medical Science
dc.rightsAcesso Aberto
dc.subjectBacterias gram-positivas
dc.subjectOntologias (Recuperação da informação)
dc.subjectSimulação (Computadores)
dc.subjectLinfadenite caseosa em caprino
dc.titleLeader gene of corynebacterium pseudotuberculosis may be useful in vaccines against caseous lymphadenitis of goats: a bioinformatics approach
dc.typeArtigo de periódico
local.citation.epage1324
local.citation.issue8
local.citation.spage1317
local.citation.volume80
local.description.resumoWe conducted an in silico analysis to search for important genes in the pathogenesis of Caseous Lymphadenitis (CL), with prospects for use in formulating effective vaccines against this disease. For this, we performed a survey of proteins expressed by Corynebacterium pseudotuberculosis, using protein sequences collected from the NCBI GenPept database and the keywords “caseous lymphadenitis” and “Corynebacterium pseudotuberculosis” and “goats”. A network was developed using the STRING 10 database, with a confidence score of 0.900. For every gene interaction identified, we summed the interaction score of each gene, generating a combined association score to obtain a single score named weighted number of links (WNL). Genes with the highest WNL were named “leader genes”. Ontological analysis was extracted from the STRING database through Kyoto Encyclopedia of Genes and Genomes (KEGG) database. A search in the GenPept database revealed 2,124 proteins. By using and plotting with STRING 10, we then developed an in silico network model comprised of 1,243 genes/proteins interconnecting through 3,330 interactions. The highest WNL values were identified in the rplB gene, which was named the leader gene. Our ontological analysis shows that this protein acts effectively mainly on Metabolic pathways and Biosynthesis of secondary metabolites. In conclusion, the in silico analyses showed that rplB has good potential for vaccine development. However, functional assays are needed to make sure that this protein can potentially induce both humoral and cellular immune responses against C. pseudotuberculosis in goats.
local.identifier.orcidhttps://orcid.org/ 0000-0001-9836-4117
local.publisher.countryBrasil
local.publisher.departmentICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS
local.publisher.initialsUFMG
local.url.externahttps://pubmed.ncbi.nlm.nih.gov/29937460/

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