Modifying internal organization and surface morphology of siRNA lipoplexes by sodium alginate addition for efficient siRNA delivery

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dc.creatorDanielle Campiol Arruda
dc.creatorArmando da Silva Cunha Júnior
dc.creatorÂngelo Malachias de Souza
dc.creatorVirginie Escriou
dc.creatorIsmael José Gonzalez
dc.creatorStéphanie Finet
dc.creatorLuis Cordova
dc.creatorValérie Trichet
dc.creatorGracielle Ferreira Andrade
dc.creatorCéline Hoffmann
dc.creatorPascal Bigey
dc.creatorWaldemar Augusto de Almeida Macedo
dc.date.accessioned2023-03-17T19:21:11Z
dc.date.accessioned2025-09-09T00:43:10Z
dc.date.available2023-03-17T19:21:11Z
dc.date.issued2019
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.identifier.doihttps://doi.org/10.1016/j.jcis.2019.01.043
dc.identifier.issn1095-7103
dc.identifier.urihttps://hdl.handle.net/1843/51013
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofJournal of Colloid and Interface Science
dc.rightsAcesso Restrito
dc.subjectÁcido ribonucleico
dc.subjectNanopartículas
dc.subject.otherRNA interference
dc.subject.otherCationic lipid
dc.subject.otherNanoparticle
dc.subject.otherDelivery system
dc.subject.otherAnionic polymer
dc.titleModifying internal organization and surface morphology of siRNA lipoplexes by sodium alginate addition for efficient siRNA delivery
dc.typeArtigo de periódico
local.citation.epage353
local.citation.spage342
local.citation.volume540
local.description.resumoVectorized small interfering RNAs (siRNAs) are widely used to induce specific mRNA degradation in the intracellular compartment of eukaryotic cells. Recently, we developed efficient cationic lipid-based siRNA vectors (siRNA lipoplexes or siLex) containing sodium alginate (Nalg-siLex) with superior efficiency and stability properties than siLex. In this study, we assessed the physicochemical and some biological properties of Nalg-siLex compared to siLex. While no significant differences in size, ζ potential and siRNA compaction were detected, the addition of sodium alginate modified the particle morphology, producing smoother and heterogeneous particles characterized by transmission electron microscopy. We also noted that Nalg-siLex have surface differences observed by X-ray photoelectron spectroscopy. These differences could arise from an internal reorganization of components induced by the addition of sodium alginate, that is indicated by Small-Angle X-ray Scattering results. Moreover, Nalg-siLex did not trigger significant hepatotoxicity nor inflammatory cytokine secretion compared to siLex. Taken together these results suggest that sodium alginate played a key role by structuring and reinforcing siRNA lipoplexes, leading to more stable and efficient delivery vector.
local.identifier.orcidhttps://orcid.org/0000-0001-9838-728X
local.identifier.orcidhttps://orcid.org/0000-0002-1161-8936
local.identifier.orcidhttps://orcid.org/0000-0002-8703-4283
local.identifier.orcidhttps://orcid.org/0000-0001-5686-9301
local.identifier.orcidhttps://orcid.org/0000-0002-9036-2835
local.identifier.orcidhttps://orcid.org/0000-0003-4183-9032
local.identifier.orcidhttps://orcid.org/0000-0001-9038-1254
local.identifier.orcidhttps://orcid.org/0000-0001-9658-6373
local.identifier.orcidhttps://orcid.org/0000-0002-6795-8569
local.identifier.orcidhttps://orcid.org/0000-0001-5451-9965
local.publisher.countryBrasil
local.publisher.departmentFAR - DEPARTAMENTO DE PRODUTOS FARMACÊUTICOS
local.publisher.departmentICX - DEPARTAMENTO DE FÍSICA
local.publisher.initialsUFMG
local.url.externahttps://www.sciencedirect.com/science/article/pii/S0021979719300542

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