Yellow fever virus genotyping tool and investigation of suspected adverse events following yellow fever vaccination

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dc.creatorIzabela Maurício de Rezende
dc.creatorPedro Augusto Alves
dc.creatorMatheus Soares Arruda
dc.creatorAndreza Parreiras Gonçalves
dc.creatorGabriela Fernanda Garcia Oliveira
dc.creatorLeonardo Soares Pereira
dc.creatorMaria Rita Teixeira Dutra
dc.creatorAna Carolina Campi-Azevedo
dc.creatorValéria Valim
dc.creatorRenata Tourinho Santos
dc.creatorJaquelline Germano de Oliveira
dc.creatorCarlos Eduardo Calzavara
dc.creatorRodrigo Fabiano do Carmo Said
dc.creatorErna Geessien Kroon
dc.creatorOlindo Assis Martins-Filho
dc.creatorAndrea Teixeira-Carvalho
dc.creatorBetânia Paiva Drumond
dc.date.accessioned2025-02-17T19:36:45Z
dc.date.accessioned2025-09-08T23:11:37Z
dc.date.available2025-02-17T19:36:45Z
dc.date.issued2019-12-04
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.3390/vaccines7040206
dc.identifier.issn2076-393X
dc.identifier.urihttps://hdl.handle.net/1843/80160
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofVaccines
dc.rightsAcesso Aberto
dc.subjectFebre amarela
dc.subjectVírus da Febre Amarela
dc.subjectVacinação
dc.subjectImunização
dc.subjectInfecções por Arbovirus
dc.subjectEfeitos Colaterais e Reações Adversas Relacionados a Medicamentos
dc.subject.otherYellow Fever virus
dc.subject.otherYFV-17DD
dc.subject.otherAdverse event
dc.subject.otherYellow Fever
dc.subject.otherVaccination
dc.subject.otherGenotyping
dc.subject.otherWild-type virus
dc.subject.otherVaccine
dc.subject.otherArboviruses
dc.subject.otherImmunization
dc.titleYellow fever virus genotyping tool and investigation of suspected adverse events following yellow fever vaccination
dc.typeArtigo de periódico
local.citation.epage10
local.citation.issue4
local.citation.spage1
local.citation.volume7
local.description.resumoThe yellow fever (YF) vaccine consists of an attenuated virus, and despite its relative safety, some adverse events following YF vaccination have been described. At the end of 2016, Brazil experienced the most massive sylvatic yellow fever outbreak over the last 70 years and an intense campaign of YF vaccination occurred in Minas Gerais state in Southeast Brazil from 2016 to 2018. The present study aimed to develop a genotyping tool and investigate 21 cases of suspected adverse events following YF vaccination. Initial in silico analyses were performed using partial NS5 nucleotide sequences to verify the discriminatory potential between wild-type and vaccine viruses. Samples from patients were screened for the presence of the YFV RNA, using 50UTR as the target, and then used for amplification of partial NS5 gene amplification, sequencing, and phylogenetic analysis. Genotyping indicated that 17 suspected cases were infected by the wild-type yellow fever virus, but four cases remained inconclusive. The genotyping tool was efficient in distinguishing the vaccine from wild-type virus, and it has the potential to be used for the differentiation of all yellow fever virus genotypes.
local.identifier.orcidhttps://orcid.org/0000-0003-2616-6300
local.identifier.orcidhttps://orcid.org/0000-0002-9659-5621
local.identifier.orcidhttps://orcid.org/0000-0001-5869-2183
local.identifier.orcidhttps://orcid.org/0000-0002-4256-247X
local.identifier.orcidhttps://orcid.org/0000-0002-5426-0655
local.identifier.orcidhttps://orcid.org/0000-0001-6306-3605
local.identifier.orcidhttps://orcid.org/0000-0002-1208-3179
local.identifier.orcidhttps://orcid.org/0000-0002-0487-8748
local.identifier.orcidhttps://orcid.org/0000-0002-1406-6401
local.identifier.orcidhttps://orcid.org/0000-0003-2200-6166
local.identifier.orcidhttps://orcid.org/0000-0003-2721-3826
local.identifier.orcidhttps://orcid.org/0000-0002-5494-4889
local.identifier.orcidhttps://orcid.org/0000-0003-2814-5183
local.identifier.orcidhttps://orcid.org/0000-0002-4049-4055
local.publisher.countryBrasil
local.publisher.departmentICB - DEPARTAMENTO DE MICROBIOLOGIA
local.publisher.initialsUFMG
local.url.externahttps://www.mdpi.com/2076-393X/7/4/206

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