Tumor necrosis factor, but not neutrophils, alters the metabolic profile in acute experimental arthritis

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dc.creatorMarina Chaves de Oliveira
dc.creatorLuciana Padua Tavares
dc.creatorJuliana Priscila Vago da Silva
dc.creatorNathália Vieira Batista
dc.creatorCelso Martins Queiroz Junior
dc.creatorAngelica Thomaz Vieira
dc.creatorGustavo Batista de Menezes
dc.creatorLirlândia Pires de Sousa
dc.creatorFons Van De Loo
dc.creatorMauro Martins Teixeira
dc.creatorFlávio Almeida Amaral
dc.creatorAdaliene Versiani Matos Ferreira
dc.date.accessioned2023-05-23T21:05:38Z
dc.date.accessioned2025-09-09T00:49:09Z
dc.date.available2023-05-23T21:05:38Z
dc.date.issued2016
dc.format.mimetypepdf
dc.identifier.doihttp://dx.doi.org/10.1371/journal.pone.0146403
dc.identifier.issn1932-6203
dc.identifier.urihttps://hdl.handle.net/1843/53841
dc.languagepor
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofPlos one
dc.rightsAcesso Aberto
dc.subjectFatores de Necrose Tumoral
dc.subjectArtrite
dc.subjectNeutrófilos
dc.subject.otherTumor Necrosis Factors
dc.subject.otherArthritis
dc.subject.otherNeutrophils
dc.titleTumor necrosis factor, but not neutrophils, alters the metabolic profile in acute experimental arthritis
dc.title.alternativeFator de necrose tumoral, mas não neutrófilos, altera o perfil metabólico na artrite experimental aguda
dc.typeArtigo de periódico
local.citation.issue1
local.citation.volume11
local.description.resumoMetabolic alterations are associated with arthritis apart from obesity. However, it is still unclear which is the underlying process behind these metabolic changes. Here, we investigate the role of tumor necrosis factor (TNF) in this process in an acute model of antigen-induced arthritis (AIA). Immunized male BALB/c mice received an intra-articular injection of PBS (control) or methylated bovine serum albumin (mBSA) into their knees, and were also pre-treated with different drugs: Etanercept, an anti-TNF drug, DF2156A, a CXCR1/2 receptor antagonist, or a monoclonal antibody RB6-8C5 to deplete neutrophils. Local challenge with mBSA evoked an acute neutrophil influx into the knee joint, and enhanced the joint nociception, along with a transient systemic metabolic alteration (higher levels of glucose and lipids, and altered adipocytokines). Pre-treatment with the conventional biological Etanercept, an inhibitor of TNF action, ameliorated the nociception and the acute joint inflammation dominated by neutrophils, and markedly improved many of the altered systemic metabolites (glucose and lipids), adipocytokines and PTX3. However, the lessening of metabolic changes was not due to diminished accumulation of neutrophils in the joint by Etanercept. Reduction of neutrophil recruitment by pre-treating AIA mice with DF2156A, or even the depletion of these cells by using RB6-8C5 reduced all of the inflammatory parameters and hypernociception developed after AIA challenge, but could not prevent the metabolic changes. Therefore, the induction of joint inflammation provoked acute metabolic alterations which were involved with TNF. We suggest that the role of TNF in arthritis-associated metabolic changes is not due to local neutrophils, which are the major cells present in this model, but rather due to cytokines.
local.identifier.orcidhttps://orcid.org/0000-0002-7423-9802
local.identifier.orcidhttps://orcid.org/0000-0002-5319-0210
local.identifier.orcidhttps://orcid.org/0000-0001-8188-3738
local.identifier.orcidhttps://orcid.org/0000-0002-7884-7709
local.identifier.orcidhttps://orcid.org/0000-0002-4556-7671
local.identifier.orcidhttps://orcid.org/0000-0002-1042-9762
local.identifier.orcidhttps://orcid.org/0000-0002-5851-099X
local.identifier.orcidhttps://orcid.org/0000-0002-6944-3008
local.identifier.orcidhttps://orcid.org/0000-0002-1695-0612
local.identifier.orcidhttps://orcid.org/0000-0003-2256-8652
local.publisher.countryBrasil
local.publisher.departmentENF - DEPARTAMENTO DE NUTRIÇÃO
local.publisher.departmentFAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICAS
local.publisher.departmentICB - DEPARTAMENTO DE BIOQUÍMICA E IMUNOLOGIA
local.publisher.departmentICB - DEPARTAMENTO DE FARMACOLOGIA
local.publisher.departmentICB - DEPARTAMENTO DE MORFOLOGIA
local.publisher.initialsUFMG
local.url.externahttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0146403#authcontrib

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