Neutrophil extracellular traps aggravate apical periodontitis by stimulating osteoclast formation

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dc.creatorJoão Luiz Guilherme Neto
dc.creatorSandra Yasuyo Fukada Alves
dc.creatorLucas Gabriel Rodrigues Venturini
dc.creatorAyda Henriques Schneider
dc.creatorThaise Mayumi Taira
dc.creatorLetícia Fernanda Duffles Rodrigues
dc.creatorFlávio Protásio Veras
dc.creatorSicília Rezende Oliveira
dc.creatorTarcília Aparecida da Silva
dc.creatorFernando de Queiroz Cunha
dc.date.accessioned2025-05-14T19:21:15Z
dc.date.accessioned2025-09-08T22:56:40Z
dc.date.available2025-05-14T19:21:15Z
dc.date.issued2023-11
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPESP - Fundação de Amparo à Pesquisa do Estado de São Paulo
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.1016/j.joen.2023.07.027
dc.identifier.issn1878-3554
dc.identifier.urihttps://hdl.handle.net/1843/82274
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofJournal of Endodontics
dc.rightsAcesso Restrito
dc.subjectPeriapical periodontitis
dc.subjectBone resorption
dc.subjectExtracellular traps
dc.subjectNeutrophils
dc.subjectOsteoclasts
dc.subjectEvaluation study
dc.subjectDeoxyribonuclease I
dc.subjectInfections
dc.subjectGene expression
dc.subjectTartrate-resistant acid phosphatase
dc.subjectRANK ligand
dc.subject.otherApical periodontitis
dc.subject.otherBone resorption
dc.subject.otherNeutrophil extracellular traps
dc.subject.otherNeutrophils
dc.subject.otherOsteoclasts
dc.titleNeutrophil extracellular traps aggravate apical periodontitis by stimulating osteoclast formation
dc.typeArtigo de periódico
local.citation.epage1521
local.citation.issue11
local.citation.spage1514
local.citation.volume49
local.description.resumoIntroduction: Neutrophil extracellular traps (NETs) have been described as structures composed of DNA and proteins, such as elastase and myeloperoxidase, that are able to kill bacteria extracellularly. The aim of the present study was to evaluate the role of NETs in bone resorption observed in pulp infection-induced apical periodontitis in mice. Methods: Apical periodontitis was experimentally induced by exposing the dental pulp of the mandibular first molar of mice to the oral microenvironment. The expression of NETs was evaluated by immunofluorescence in mice and biopsies of apical periodontitis. Mice were treated with vehicle or DNase I to degrade NETs, and the samples were collected after 7 days. The size of the apical lesion and the osteoclast number were determined in hematoxylin-eosin- and tartrate-resistant acid phosphatase-stained sections, respectively. Osteoclast differentiation and function markers were evaluated by quantitative polymerase chain reaction. The level of NETs in the serum was determined by the myeloperoxidase-DNA PicoGreen assay. Results: We first confirmed the presence of neutrophils and NETs at the site of the lesion in mice and in biopsies of patients with apical periodontitis. The treatment of mice with DNase I reduced the level of NETs in the serum and led to a reduction in apical lesion size and alveolar bone resorption. This effect was associated with a reduction of local inflammatory infiltrate and a reduced number of osteoclasts. We found that the increased expression of Acp5, Ctsk, and Rankl genes associated with osteoclast formation and function were abrogated by the absence of NETs. Conclusions: Our data highlight NETs as an important player in the pathogenesis of apical periodontitis with regard to the local inflammation and consequent bone resorption after pulp infection.
local.identifier.orcidhttps://orcid.org/0000-0002-6589-4784
local.identifier.orcidhttps://orcid.org/0000-0002-7585-7709
local.identifier.orcidhttps://orcid.org/0000-0003-2474-2773
local.identifier.orcidhttps://orcid.org/0000-0003-3719-9503
local.identifier.orcidhttps://orcid.org/0000-0002-2781-9986
local.identifier.orcidhttps://orcid.org/0000-0002-6222-4064
local.identifier.orcidhttps://orcid.org/0000-0002-8164-2122
local.identifier.orcidhttps://orcid.org/0000-0001-9623-7835
local.identifier.orcidhttps://orcid.org/0000-0003-4755-1670
local.publisher.countryBrasil
local.publisher.departmentFAO - DEPARTAMENTO DE CLÍNICA
local.publisher.initialsUFMG
local.url.externahttps://www.sciencedirect.com/science/article/pii/S0099239923005034?via%3Dihub

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