DNA methylation profile of genes related to immune response in generalized periodontitis

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dc.creatorAndréa Mara de Oliveira Azevedo
dc.creatorLuiz Paulo Carvalho Rocha
dc.creatorSimone Angélica de Faria Amormino Carvalho
dc.creatorCarolina Cavalieri Gomes
dc.creatorWalderez Ornelas Dutra
dc.creatorRicardo Santiago Gomez
dc.creatorJosé Eustáquio da Costa
dc.creatorPaula Rocha Moreira
dc.date.accessioned2025-08-27T19:36:14Z
dc.date.accessioned2025-09-09T00:28:45Z
dc.date.available2025-08-27T19:36:14Z
dc.date.issued2020-06
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.1111/jre.12726
dc.identifier.issn1600-0765
dc.identifier.urihttps://hdl.handle.net/1843/84633
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofJournal of Periodontal Research
dc.rightsAcesso Restrito
dc.subjectEpigenomics
dc.subjectMethylation
dc.subjectPeriodontitis
dc.subjectAssociation
dc.subjectGenes
dc.subject.otherEpigenetic
dc.subject.otherImmunology
dc.subject.otherMethylation
dc.subject.otherPeriodontitis
dc.titleDNA methylation profile of genes related to immune response in generalized periodontitis
dc.typeArtigo de periódico
local.citation.epage431
local.citation.issue3
local.citation.spage426
local.citation.volume55
local.description.resumoBackground and objective: Epigenetic events, as the DNA methylation, may be related to development of inflammatory diseases. Due to the important role of host's response in the pathogenesis of periodontitis, the purpose of the present study was to investigate the methylation profile of genes related to immune response in gingival tissues from patients with generalized periodontitis (GP) compared to healthy individuals. Methods: Gingival tissues were collected from 20 individuals with GP and 20 healthy individuals. Genomic DNA was extracted and submitted to enzymatic digestions. An initial screening using a panel of genes involved with the response immune was performed in pools containing six samples of each group. Genes that presented different levels of methylation between the groups were selected for individual assays for validation. Results: The array results showed an unmethylated profile in the majority of genes evaluated in both groups. MALT1, LTB, and STAT5 genes presented a profile of partial methylation in the control compared with GP group. Validation individual assays using a larger number of samples (n = 20, each group) confirmed the hypomethylation of STAT5 in the GP group compared with control group (P < .001). Conclusion: Generalized periodontitis is associated with hypomethylation of the STAT5 gene. Further studies are necessary to evaluate the functional impact these findings.
local.identifier.orcidhttps://orcid.org/0000-0002-6074-5093
local.identifier.orcidhttps://orcid.org/0000-0003-1580-4995
local.identifier.orcidhttps://orcid.org/0000-0002-7586-9996
local.identifier.orcidhttps://orcid.org/0000-0001-8770-8009
local.identifier.orcidhttps://orcid.org/0000-0003-1078-6281
local.publisher.countryBrasil
local.publisher.departmentFAO - DEPARTAMENTO DE CLÍNICA
local.publisher.departmentICB - DEPARTAMENTO DE MORFOLOGIA
local.publisher.departmentICB - DEPARTAMENTO DE PATOLOGIA
local.publisher.initialsUFMG
local.url.externahttps://onlinelibrary.wiley.com/doi/10.1111/jre.12726

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