DNA methylation pattern of apoptosis-related genes in ameloblastoma

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dc.creatorSara Ferreira dos Santos Costa
dc.creatorNubia Braga Pereira
dc.creatorKaruza Maria Alves Pereira
dc.creatorKelma Campos
dc.creatorWagner Henriques de Castro
dc.creatorMarina Gonçalves Diniz
dc.creatorCarolina Cavalieri Gomes
dc.creatorRicardo Santiago Gomez
dc.date.accessioned2025-06-25T19:29:16Z
dc.date.accessioned2025-09-09T01:29:52Z
dc.date.available2025-06-25T19:29:16Z
dc.date.issued2017-09
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.1111/odi.12661
dc.identifier.issn1601-0825
dc.identifier.urihttps://hdl.handle.net/1843/83133
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofOral Diseases
dc.rightsAcesso Restrito
dc.subjectBcl-2-like protein 11
dc.subjectDNA methylation
dc.subjectReceptors, Tumor Necrosis Factor, Member 25
dc.subjectAmeloblastoma
dc.subjectApoptosis
dc.subjectGenes
dc.subjectEvaluation study
dc.subjectDental sac
dc.subjectGene expression
dc.subjectTranscription, genetic
dc.subject.otherBcl-2-like protein 11
dc.subject.otherDNA methylation
dc.subject.otherTNFRSF25
dc.subject.otherAmeloblastoma
dc.subject.otherApoptosis
dc.titleDNA methylation pattern of apoptosis-related genes in ameloblastoma
dc.typeArtigo de periódico
local.citation.epage783
local.citation.issue6
local.citation.spage779
local.citation.volume23
local.description.resumoObjectives: DNA methylation is an important mechanism of gene control expression, and it has been poorly addressed in odontogenic tumours. On this basis, we aimed to assess the methylation pattern of 22 apoptosis-related genes in solid ameloblastomas. Materials and methods: Ameloblastoma fresh samples (n = 10) and dental follicles (n = 8) were included in the study. The percentage fraction of methylated and unmethylated DNA promoter of 22 apoptosis-related genes was determined using enzymatic restriction digestion and quantitative real-time PCR (qPCR) array. The relative expressions of the genes that showed the most discrepant methylation profile between tumours and controls were analysed by reverse-transcription quantitative PCR (RT-qPCR). Results: Lower methylation percentages of TNFRSF25 (47.2%) and BCL2L11 (33.2%) were observed in ameloblastomas compared with dental follicles (79.3% and 59.5%, respectively). The RT-qPCR analysis showed increased expression of BCL2L11 in ameloblastomas compared with dental follicles, in agreement with the methylation analysis results, while there was no difference between the expression levels of TNFRSF25 between both groups. Conclusions: On the basis of our results, the transcription of the apoptosis-related gene BCL2L11 is possibly regulated by promoter DNA methylation in ameloblastoma. The biological significance of this finding in ameloblastoma pathobiology remains to be clarified.
local.identifier.orcidhttps://orcid.org/0000-0001-8770-8009
local.identifier.orcidhttps://orcid.org/0000-0002-2880-6466
local.identifier.orcidhttps://orcid.org/0000-0002-6714-3124
local.identifier.orcidhttps://orcid.org/0000-0001-5150-9227
local.identifier.orcidhttps://orcid.org/0000-0003-2745-2878
local.identifier.orcidhttps://orcid.org/0000-0002-4212-1172
local.identifier.orcidhttps://orcid.org/0000-0003-1580-4995
local.publisher.countryBrasil
local.publisher.departmentFAO - DEPARTAMENTO DE CLÍNICA
local.publisher.departmentICB - DEPARTAMENTO DE MORFOLOGIA
local.publisher.departmentICB - DEPARTAMENTO DE PATOLOGIA
local.publisher.initialsUFMG
local.url.externahttps://onlinelibrary.wiley.com/doi/10.1111/odi.12661

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