Preconditioning methods to improve mesenchymal stromal cell-derived extracellular vesicles in bone regeneration: a systematic review

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dc.creatorFernanda Campos Hertel
dc.creatorAline Silvestrini da Silva
dc.creatorAdriano de Paula Sabino
dc.creatorFabrício Luciani Valente
dc.creatorEmily Correna Carlo Reis
dc.date.accessioned2025-04-11T15:00:04Z
dc.date.accessioned2025-09-09T00:34:12Z
dc.date.available2025-04-11T15:00:04Z
dc.date.issued2022-05
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.3390/biology11050733
dc.identifier.issn2079-7737
dc.identifier.urihttps://hdl.handle.net/1843/81480
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofBiology
dc.rightsAcesso Aberto
dc.subjectComunicação celular
dc.subjectMeios de cultivo condicionados
dc.subjectExossomos
dc.subjectOsteogênese
dc.subject.otherCell communication
dc.subject.otherConditioned medium
dc.subject.otherExosomes
dc.subject.otherMicroenvironment
dc.subject.otherOsteogenesis
dc.titlePreconditioning methods to improve mesenchymal stromal cell-derived extracellular vesicles in bone regeneration: a systematic review
dc.typeArtigo de periódico
local.citation.epage29
local.citation.issue5
local.citation.spage1
local.citation.volume11
local.description.resumoMesenchymal stromal cells (MSCs) have long been used in research for bone regeneration, with evidence of their beneficial properties. In the segmental area of MSC-based therapies, MSC-derived extracellular vesicles (EVs) have also shown great therapeutic effects in several diseases, including bone healing. This study aimed to assess whether the conditioning of MSCs improves the therapeutic effects of their derived extracellular vesicles for bone regeneration. Electronic research was performed until February 2021 to recover the studies in the following databases: PubMed, Scopus, and Web of Science. The studies were screened based on the inclusion criteria. Relevant information was extracted, including in vitro and in vivo experiments, and the animal studies were evaluated for risk of bias by the SYRCLE tool. A total of 463 studies were retrieved, and 18 studies met the inclusion criteria (10 studies for their in vitro analysis, and 8 studies for their in vitro and in vivo analysis). The conditioning methods reported included: osteogenic medium; dimethyloxalylglycine; dexamethasone; strontium-substituted calcium silicate; hypoxia; 3D mechanical microenvironment; and the overexpression of miR-375, bone morphogenetic protein-2, and mutant hypoxia-inducible factor-1α. The conditioning methods of MSCs in the reported studies generate exosomes able to significantly promote bone regeneration. However, heterogeneity regarding cell source, conditioning method, EV isolation and concentration, and defect model was observed among the studies. The different conditioning methods reported in this review do improve the therapeutic effects of MSC-derived EVs for bone regeneration, but they still need to be addressed in larger animal models for further clinical application.
local.publisher.countryBrasil
local.publisher.departmentFAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICAS
local.publisher.initialsUFMG
local.url.externahttps://www.mdpi.com/2079-7737/11/5/733

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