Avaliação da eficiência de "kits" destinados à detecção de resíduos de antimicrobianos em leite de vacas tratadas com ivermectina e abamectina

Abstract

The maximum residues limits of veterinary drugs in milk and dairy products are importantparameters for international market and to assure safety levels of residues for the consumer.Therefore, to obtain safe milk, it is necessary the utilization of safe diagnostic methods. Theobjectives of this experiment were to evaluate the efficiency of some analytical kits (SNAP® -Beta-lactam test kit, DELVOTEST SP®, CH ATK® single test P&S, BETA STAR®, CHARMMRL® Test, and CHARM SL® Test) used to detect inhibitors residues in milk obtained fromcows administered with ivermectin and abamectin, and to evaluate the curve of excretion ofthese drugs in the milk. Three groups of cows, with three cows each, were treated with,respectively, ivermectin, abamectin, and the third group was used as a control. Samples wereobtained before treatment, in the four following days, and weekly until the 38th day. Each milksample was analyzed for composition, somatic cell count, bacterial count, presence of inhibitorresidues, and pools of samples for the different groups in the collect days were analyzed byHPLC to determine levels of ivermectin and abamectin. There was no cross-reaction in theresults obtained with the kits for antibiotics. The metabolic excretion curve for avermectinsshows that these drugs were eliminated in the milk until the 7th day after treatment