Biological and Molecular Effects of Trypanosoma cruzi Residence in a LAMP-Deficient Intracellular Environment

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dc.creatorAnny Carolline Silva Oliveira
dc.creatorHélida Monteiro de Andrade
dc.creatorLuciana de Oliveira Andrade
dc.creatorLuisa Rezende
dc.creatorVladimir Gorshkov
dc.creatorMarcella Nunes Melo-Braga
dc.creatorWeslley Fernandes-Braga
dc.creatorJorge Luís de Melo Guadalupe
dc.creatorGustavo Batista de Menezes
dc.creatorFrank Kjeldsen
dc.creatorThiago Verano-Braga
dc.date.accessioned2023-10-30T21:06:07Z
dc.date.accessioned2025-09-09T01:23:05Z
dc.date.available2023-10-30T21:06:07Z
dc.date.issued2022-01-06
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.3389/fcimb.2021.788482
dc.identifier.issn2235-2988
dc.identifier.urihttps://hdl.handle.net/1843/60284
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofFrontiers in Celular and Infection Microbiology
dc.rightsAcesso Aberto
dc.subjectTrypanosoma cruzi
dc.subjectDoença de Chagas
dc.subjectProteínas
dc.subjectParasitos
dc.subject.otherTrypanosoma cruzi
dc.subject.otherChagas disease
dc.subject.otherLAMP proteins
dc.subject.otherParasite invasion
dc.subject.otherIntracellular environment
dc.subject.otherProteomic analysis
dc.subject.otherTMT (tandem mass tags)
dc.titleBiological and Molecular Effects of Trypanosoma cruzi Residence in a LAMP-Deficient Intracellular Environment
dc.typeArtigo de periódico
local.citation.epage18
local.citation.spage788482
local.citation.volume11
local.description.resumoTrypanosoma cruzi invades non-professional phagocytic cells by subverting their membrane repair process, which is dependent on membrane injury and cell signaling, intracellular calcium increase, and lysosome recruitment. Cells lacking lysosome-associated membrane proteins 1 and 2 (LAMP1 and LAMP2) are less permissive to parasite invasion but more prone to parasite intracellular multiplication. Several passages through a different intracellular environment can significantly change T. cruzi’s gene expression profile. Here, we evaluated whether one single passage through LAMP-deficient (KO) or wild-type (WT) fibroblasts, thus different intracellular environments, could influence T. cruzi Y strain trypomastigotes’ ability to invade L6 myoblasts and WT fibroblasts host cells. Parasites released from LAMP2 KO cells (TcY-L2−/−) showed higher invasion, calcium signaling, and membrane injury rates, for the assays in L6 myoblasts, when compared to those released from WT (TcY-WT) or LAMP1/2 KO cells (TcY-L1/2−/−). On the other hand, TcY-L1/2−/− showed higher invasion, calcium signaling, and cell membrane injury rates, for the assays in WT fibroblasts, compared to TcY-WT and TcY-L1/2−/−. Albeit TcY-WT presented an intermediary invasion and calcium signaling rates, compared to the others, in WT fibroblasts, they induced lower levels of injury, which reinforces that signals mediated by surface membrane protein interactions also have a significant contribution to trigger host cell calcium signals. These results clearly show that parasites released from WT or LAMP KO cells are distinct from each other. Additionally, these parasites’ ability to invade the cell may be distinct depending on which cell type they interact with. Since these alterations most likely would reflect differences among parasite surface molecules, we also evaluated their proteome. We identified few protein complexes, membrane, and secreted proteins regulated in our dataset. Among those are some members of MASP, mucins, trans-sialidases, and gp63 proteins family, which are known to play an important role during parasite infection and could correlate to TcY-WT, TcY-L1/2−/−, and TcY-L2−/− biological behavior.
local.identifier.orcidhttps://orcid.org/0000-0003-2823-4530
local.publisher.countryBrasil
local.publisher.departmentFAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICAS
local.publisher.departmentFARMACIA - FACULDADE DE FARMACIA
local.publisher.departmentICB - DEPARTAMENTO DE BIOQUÍMICA E IMUNOLOGIA
local.publisher.departmentICB - DEPARTAMENTO DE FISIOLOGIA E BIOFÍSICA
local.publisher.departmentICB - DEPARTAMENTO DE MORFOLOGIA
local.publisher.departmentICB - DEPARTAMENTO DE PARASITOLOGIA
local.publisher.initialsUFMG
local.url.externahttps://www.frontiersin.org/articles/10.3389/fcimb.2021.788482/full

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