Anti-mitochondrial tryparedoxin peroxidase monoclonal antibody-based immunohistochemistry for diagnosis of cutaneous leishmaniasis

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dc.creatorMariana Lourenço Freire
dc.creatorFelipe Dutra Rego
dc.creatorKarine Ferreira Lopes
dc.creatorLucélia Antunes Coutinho
dc.creatorRafaella Fortini Queiroz Grenfell
dc.creatorDaniel Moreira Avelar
dc.creatorGlaucia Fernandes Cota
dc.creatorMarcelo Antônio Pascoal-Xavier
dc.creatorEdward Oliveira
dc.date.accessioned2023-12-07T21:39:57Z
dc.date.accessioned2025-09-09T00:17:10Z
dc.date.available2023-12-07T21:39:57Z
dc.date.issued2022-02-28
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.description.sponsorshipOutra Agência
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.3389/fmicb.2021.790906
dc.identifier.issn1664-302X
dc.identifier.urihttps://hdl.handle.net/1843/61858
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofFrontiers in Microbiology
dc.rightsAcesso Aberto
dc.subjectLeishmaniose cutânea
dc.subjectImuno-Histoquímica
dc.subjectAnticorpos Monoclonais
dc.subjectTécnicas de Laboratório Clínico
dc.subject.otherCutaneous leishmaniasis
dc.subject.otherImmunohistochemistry
dc.subject.otherDiagnosis
dc.subject.otherMonoclonal antibody
dc.subject.othermTXNPx
dc.titleAnti-mitochondrial tryparedoxin peroxidase monoclonal antibody-based immunohistochemistry for diagnosis of cutaneous leishmaniasis
dc.typeArtigo de periódico
local.citation.epage14
local.citation.spage1
local.citation.volume12
local.description.resumoCutaneous leishmaniasis (CL) remains a globally spreading public health problem. Among Latin America countries, Brazil has the greatest number of recorded CL cases with several Leishmania species being associated with human cases. Laboratory diagnosis is one of the major challenges to disease control due to the low accuracy of parasitological techniques, the restricted use of molecular techniques, and the importance of differential diagnosis with regard to several dermatological and systemic diseases. In response, we have developed and validated an immunohistochemistry (IHC) technique for CL diagnosis using anti-mTXNPx monoclonal antibody (mAb). Recombinant Leishmania–mTXNPx was produced and used as an immunogen for mAb production through the somatic hybridization technique. The viability of mAb labeling of Leishmania amastigotes was tested by IHC performed with skin biopsies from hamsters experimentally infected with Leishmania amazonensis, Leishmania braziliensis, and Leishmania guyanensis. The enzymes horseradish peroxidase (IHC-HRP) and alkaline phosphatase (IHC-AP), both biotin-free polymer detection systems, were used in the standardization step. The IHC was further validated with skin biopsies from 49 CL patients diagnosed by clinical examination and quantitative real-time polymerase chain reaction and from 37 patients presenting other dermatological infectious diseases. Other parasitological techniques, such as direct examination and culture, were also performed for confirmed CL patients. Histopathology and IHC were performed for all included patients. Overall, the highest sensitivity was observed for IHC-AP (85.7%), followed by IHC-HRP (79.6%), direct examination (77.6%), histopathological examination (HE; 65.3%), and in vitro culture (49%). Only IHC and HE presented specificity over 90% and were able to detect CL patients regardless of parasite burden (odds ratio > 1.94; 95%CI: 0.34–11.23). A significant increase in positivity rates was observed when IHC-AP was combined with direct examination (95.9%) and HE (93.9%). The IHC techniques evaluated in here detected the main Leishmania species causing CL in Brazil and can support diagnostic strategies for controlling this neglected disease, especially if used in combination with other approaches for an integrative laboratorial diagnosis.
local.identifier.orcidhttps://orcid.org/0000-0003-0538-7403
local.publisher.countryBrasil
local.publisher.departmentHCL - HOSPITAL DAS CLINICAS
local.publisher.departmentMED - DEPARTAMENTO DE ANATOMIA PATOLÓGICA E MEDICINA LEGAL
local.publisher.initialsUFMG
local.url.externahttps://www.frontiersin.org/articles/10.3389/fmicb.2021.790906/full

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