Cohesin subunits, STAG1 and STAG2, and cohesin regulatory factor, PDS5B, in oral squamous cells carcinomas

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dc.creatorJosiane Alves França
dc.creatorMarina Gonçalves Diniz
dc.creatorVanessa Fátima Bernardes
dc.creatorRaíssa Cristina Costa-Silva
dc.creatorRenan Pedra Souza
dc.creatorRicardo Santiago Gomez
dc.creatorCarolina Cavaliéri Gomes
dc.date.accessioned2025-07-01T19:31:40Z
dc.date.accessioned2025-09-08T23:14:28Z
dc.date.available2025-07-01T19:31:40Z
dc.date.issued2017-03
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.1111/jop.12474
dc.identifier.issn1600-0714
dc.identifier.urihttps://hdl.handle.net/1843/83257
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofJournal of Oral Pathology & Medicine
dc.rightsAcesso Restrito
dc.subjectMouth neoplasms
dc.subjectGenes
dc.subjectGene expression
dc.subjectSquamous cell carcinoma of head and neck
dc.subjectKi-67 antigen
dc.subjectTranscription, genetic
dc.subjectProteins
dc.subjectLoss of heterozygosity
dc.subjectEvaluation study
dc.subject.otherPDS5B
dc.subject.otherSTAG2
dc.subject.otherSTAG1
dc.subject.otherCell cycle genes
dc.subject.otherOral cancer
dc.titleCohesin subunits, STAG1 and STAG2, and cohesin regulatory factor, PDS5B, in oral squamous cells carcinomas
dc.typeArtigo de periódico
local.citation.epage193
local.citation.issue3
local.citation.spage188
local.citation.volume46
local.description.resumoBackground: Cohesin complex is responsible for sister chromatid cohesion. STAG1/STAG2 is part of the complex, which is regulated by PDS5B. Alterations in these genes were described in tumors. PDS5B is a negative regulator of cell proliferation. We aimed to assess molecular alterations in these genes in oral squamous cell carcinoma (OSCC) and predict their expression by the expression of 84 cell cycle genes. In addition, we investigated whether pds5b protein expression impacted ki-67 and p53 immunopositivity. Methods: We assessed loss of heterozygosity (LOH) at STAG1 and STAG2 loci in 15 OSCC using three polymorphic markers. Associations between the immunoexpression of pds5b and ki-67 and p53 were tested in 62 samples. Differences between transcriptional levels of STAG1, STAG2, and PDS5B between OSCC and normal oral mucosa (NM) were evaluated by qPCR. An 84 cell cycle genes qPCR array was carried with OSCC samples, and STAG1, STAG2, and PDS5B were independently used as response variables in multiple linear regression models. Results: Loss of heterozygosity in at least one marker was observed in three samples. pds5b, p53, and ki-67 were highly expressed, and no association was found between pds5b immunoexpression and ki-67 or p53 (P > 0.05). OSCC and NM showed similar transcriptional levels of STAG1, STAG2, and PDS5B. STAG1 and CUL3 expression seem to be related (P = 0.004). Conclusions: There is LOH at STAG1 and STAG2 loci in OSCC, but OSCC and NM showed similar transcriptional levels of STAG1, STAG2, and PDS5B. pds5b immunoexpression in OSCC was high, but it was not associated with proliferation cell index.
local.identifier.orcidhttps://orcid.org/0000-0002-6005-783X
local.identifier.orcidhttps://orcid.org/0000-0002-4212-1172
local.identifier.orcidhttps://orcid.org/0000-0003-0194-7434
local.identifier.orcidhttps://orcid.org/0000-0002-9479-4432
local.identifier.orcidhttps://orcid.org/0000-0001-8770-8009
local.identifier.orcidhttps://orcid.org/0000-0003-1580-4995
local.publisher.countryBrasil
local.publisher.departmentFAO - DEPARTAMENTO DE CLÍNICA
local.publisher.departmentICB - DEPARTAMENTO DE MORFOLOGIA
local.publisher.departmentICB - DEPARTAMENTO DE PATOLOGIA
local.publisher.initialsUFMG
local.url.externahttps://onlinelibrary.wiley.com/doi/10.1111/jop.12474

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