Effects of bisphenol A on murine salivary glands and human tumor cell lines

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dc.creatorGabriela Kelly da Silva
dc.creatorJosé Alcides Almeida de Arruda
dc.creatorTatiana Fernandes Araújo Almeida
dc.creatorSicília Rezende Oliveira
dc.creatorPaula Alves da Silva Rocha
dc.creatorRicardo Alves de Mesquita
dc.creatorZenilda de Lourdes Cardeal
dc.creatorHelvécio Costa Menezes
dc.creatorIvana Márcia Alves Diniz
dc.creatorSoraia Macari
dc.creatorAndréia Machado Leopoldino
dc.creatorTarcília Aparecida da Silva
dc.date.accessioned2025-05-22T19:57:51Z
dc.date.accessioned2025-09-09T01:28:21Z
dc.date.available2025-05-22T19:57:51Z
dc.date.issued2023-12
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.1016/j.yexmp.2023.104870
dc.identifier.issn1096-0945
dc.identifier.urihttps://hdl.handle.net/1843/82460
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofExperimental and molecular pathology
dc.rightsAcesso Aberto
dc.subjectEndocrine disruptors
dc.subjectBisphenol A compounds
dc.subjectEnvironmental pollutants
dc.subjectReceptors, estrogen
dc.subjectSalivary glands
dc.subjectCarcinoma, mucoepidermoid
dc.subjectSubmandibular gland
dc.subjectGene expression
dc.subjectImmunohistochemistry
dc.subjectOvariectomy
dc.subject.otherBisphenol A
dc.subject.otherEndocrine-disrupting chemical
dc.subject.otherEnvironmental pollutant
dc.subject.otherEstrogen receptors
dc.subject.otherSalivary glands
dc.titleEffects of bisphenol A on murine salivary glands and human tumor cell lines
dc.typeArtigo de periódico
local.citation.spage104870
local.citation.volume134
local.description.resumoBisphenol A (BPA) is an endocrine-disrupting chemical with a potential role in endocrine cancers. However, the effects of BPA on the salivary glands have been barely explored. We investigated the impact of in vivo sub-chronic exposure to BPA and its in vitro effects on human salivary gland mucoepidermoid carcinoma cell lines. Male and female mice were exposed to BPA (30 mg/kg/day). Sublingual and submandibular salivary glands from an estrogen-deficiency model were also analyzed. BPA concentration in salivary glands was evaluated by gas chromatography coupled to ion trap mass spectrometry. Immunohistochemical analysis using anti-p63 and anti-α-SMA antibodies was performed on mouse salivary gland tissues. Gene expression of estrogen receptors alpha and beta, P63 and α-SMA was quantified in mouse salivary gland and/or mucoepidermoid (UM-HMC-1 and UM-HMC-3A) cell lines. Cell viability, p63 and Ki-67 immunostaining were evaluated in vitro. BPA disrupted the tissue architecture of the submandibular and sublingual glands, particularly in female mice, and increased the expression of estrogen receptors and p63, effects that were accompanied by significant BPA accumulation in these tissues. Conversely, ovariectomy slightly impacted BPA-induced morphological changes. In vitro, BPA did not affect the proliferation of neoplastic cells, but augmented the expression of p63 and estrogen receptors. The present data highlight a potential harmful effect of BPA on salivary gland tissues, particularly in female mice, and salivary gland tumor cells. Our findings suggest that estrogen-dependent pathways may orchestrate the effects of BPA in salivary glands.
local.identifier.orcidhttps://orcid.org/0000-0001-7643-6589
local.identifier.orcidhttps://orcid.org/0000-0002-8313-4754
local.identifier.orcidhttps://orcid.org/0000-0001-9623-7835
local.identifier.orcidhttps://orcid.org/0000-0002-6599-3950
local.identifier.orcidhttps://orcid.org/0000-0002-3216-5649
local.identifier.orcidhttps://orcid.org/0000-0002-8164-2122
local.identifier.orcidhttps://orcid.org/0000-0003-3207-4007
local.identifier.orcidhttps://orcid.org/0000-0003-3427-1864
local.identifier.orcidhttps://orcid.org/0000-0003-1383-6299
local.publisher.countryBrasil
local.publisher.departmentFAO - DEPARTAMENTO DE CLÍNICA
local.publisher.departmentFAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORA
local.publisher.departmentICX - DEPARTAMENTO DE QUÍMICA
local.publisher.initialsUFMG
local.url.externahttps://www.sciencedirect.com/science/article/pii/S0014480023000217?via%3Dihub

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