Apoptosis-related gene expression can predict the response of ovarian cancer cell lines to treatment with recombinant human TRAIL alone or combined with cisplatin

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dc.creatorLetícia da Conceição Braga
dc.creatorAgnaldo Lopes da Silva Filho
dc.creatorWarne Pedro Andrade
dc.creatorRafaela de Souza Furtado
dc.creatorLuciana Maria Silva
dc.creatorNikole Gontijo Gonçalves
dc.date.accessioned2023-06-08T00:10:38Z
dc.date.accessioned2025-09-09T01:30:44Z
dc.date.available2023-06-08T00:10:38Z
dc.date.issued2020
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.6061/clinics/2020/e1492
dc.identifier.issn1980-5322
dc.identifier.urihttps://hdl.handle.net/1843/54694
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofClinics
dc.rightsAcesso Aberto
dc.subjectNeoplasias ovarianas
dc.subjectExpressão gênica
dc.subjectApoptose
dc.subject.otherOvarian cancer
dc.subject.otherGene expression
dc.subject.otherChemoresistance
dc.subject.otherApoptosis
dc.subject.otherTrail
dc.titleApoptosis-related gene expression can predict the response of ovarian cancer cell lines to treatment with recombinant human TRAIL alone or combined with cisplatin
dc.typeArtigo de periódico
local.citation.volume75
local.description.resumoOBJECTIVES: The objectives of this study were to determine the sensitivity of ovarian cancer (OC) cell lines (TOV-21G and SKOV-3) to cisplatin and to the recombinant human TRAIL (rhTRAIL), and to evaluate the expression profile of TNFRSF10B, TNFRSF10C, TP53TG5, MDM2, BAX, BCL-2 and CASPASE-8 genes and their participation in the resistance/susceptibility mechanism of these tumor cell lines. METHODS: To determine the IC50 values associated with Cisplatin and rhTRAIL, inhibition of cell growth was observed using MTT assays in two human OC cell lines (SKOV-3 and TOV-21G). The analysis of gene expression was performed using quantitative real-time polymerase chain reaction (qRT-PCR). RESULTS: Both cell lines had different susceptibility profiles to the tested drugs. In the SKOV-3 cell line, the IC50 values for cisplatin and for rhTRAIL were 270.83 ug/mL and 196.5 ng/mL, respectively. The same concentrations were used for TOV-21G. Different gene expression profiles were observed in each tested cell line. CASPASE-8 and TNFRSF10B expression levels could predict the response of both the cell lines to rhTRAIL alone or the response to a combination of rhTRAIL and cisplatin. In addition, we observed a relationship between BCL-2 and BAX expression that may be helpful in estimating the proliferation rate of the OC cell lines. CONCLUSION: SKOV-3 and TOV-21G respond differently to cisplatin and rhTRAIL exposure, and expression of CASPASE-8 and TNFRSF10B are good predictors of responses to these treatments.
local.identifier.orcidhttps://orcid.org/0000-0002-8486-7861
local.publisher.countryBrasil
local.publisher.departmentMED - DEPARTAMENTO DE GINECOLOGIA OBSTETRÍCIA
local.publisher.initialsUFMG
local.url.externahttps://www.scielo.br/j/clin/a/9ZGtfXVCX5w7K9wXRVhPYSK/?lang=en

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