Changes in cellular proliferation and plasma products are associated with liver failure

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dc.creatorJuliana Gilmelgaço
dc.creatorGustavo Batista Menezes
dc.creatorOswaldo Gonçalves Cruz
dc.creatorClaudia Lamarca Vitral
dc.creatorMarcelo Alves Pinto
dc.creatorFrederico Marianetti Soriani
dc.creatorPedro Henrique Sucupira Ferreira
dc.creatorLeonardo Assaf Pinheiro
dc.creatorYasmine Rangel Vieira
dc.creatorJaqueline Mendes de Oliveira
dc.creatorLia Laura Lewis-ximenez
dc.creatorCristina Carvalho Vianna Araújo
dc.creatorLúcio Filgueiras Pacheco-moreira
dc.date.accessioned2025-01-21T20:03:12Z
dc.date.accessioned2025-09-09T01:18:33Z
dc.date.available2025-01-21T20:03:12Z
dc.date.issued2016-11-18
dc.format.mimetypepdf
dc.identifier.doihttps://10.4254/wjh.v8.i32.1370
dc.identifier.issn1948-5182
dc.identifier.urihttps://hdl.handle.net/1843/79376
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofWorld Journal of Hepatology
dc.rightsAcesso Aberto
dc.subjectInsuficiência renal aguda
dc.subjectGenética
dc.subjectHepatite A
dc.subjectDNA
dc.subject.otherAcute liver failure
dc.subject.otherCytokines
dc.subject.otherMitochondrial DNA
dc.subject.otherCellular immune response
dc.subject.otherHepatitis A virus
dc.titleChanges in cellular proliferation and plasma products are associated with liver failure
dc.typeArtigo de periódico
local.citation.epage1383
local.citation.issue32
local.citation.spage1370
local.citation.volume8
local.description.resumoAIM To study the differences in immune response and cytokine profile between acute liver failure and self-limited acute hepatitis. METHODS Forty-six patients with self-limited acute hepatitis (AH), sixteen patients with acute liver failure (ALF), and twenty-two healthy subjects were involved in this study. The inflammatory and anti-inflammatory products in plasma samples were quantified using commercial enzyme-linked immunoassays and quantitative real-time PCR. The cellular immune responses were measured by proliferation assay using flow cytometry. The groups were divided into viral- and non-viral-induced self-limited AH and ALF. Thus, we worked with five groups: Hepatitis A virus (HAV)-induced self-limited acute hepatitis (HAV-AH), HAV-induced ALF (HAV-ALF), non-viral-induced self-limited acute hepatitis (non-viral AH), non-viral-induced acute liver failure (non-viral ALF), and healthy subjects (HC). Comparisons among HAV and non-viral-induced AH and ALF were performed. RESULTS The levels of mitochondrial DNA (mtDNA) and the cytokines investigated [interleukin (IL)-6, IL-8, IL-10, interferon gamma, and tumor necrosis factor] were significantly increased in ALF patients, independently of etiology (P < 0.05). High plasma mtDNA and IL-10 were the best markers associated with ALF [mtDNA: OR = 320.5 (95%CI: 14.42-7123.33), P < 0.0001; and IL-10: OR = 18.8 (95%CI: 1.38-257.94), P = 0.028] and death [mtDNA: OR = 12.1 (95%CI: 2.57-57.07), P = 0.002; and IL-10: OR = 8.01 (95%CI: 1.26-50.97), P = 0.027]. In the cellular proliferation assay, NKbright, NKT and regulatory T cells (TReg) predominated in virus-specific stimulation in HAV-induced ALF patients with an anergic behavior in the cellular response to mitotic stimulation. Therefore, in non-viral-induced ALF, anergic behavior of activated T cells was not observed after mitotic stimulation, as expected and as described by the literature. CONCLUSION mtDNA and IL-10 may be predictors of ALF and death. TReg cells are involved in immunological disturbance in HAV-induced ALF. Melgaço JG, Soriani FM, Sucupira PHF, Pinheiro LA, Vieira YR, de Oliveira JM, Lewis-Ximenez LL, Araújo CCV, Pacheco-Moreira LF, Menezes GB, Cruz OG, Vitral CL, Pinto MA. Changes in cellular proliferation and plasma products are associated with liver failure. World J Hepatol 2016; 8(32): 1370-1383 [PMID: 27917263 DOI: 10.4254/wjh.v8.i32.1370]
local.publisher.countryBrasil
local.publisher.departmentICB - DEPARTAMENTO DE MORFOLOGIA
local.publisher.initialsUFMG
local.url.externahttps://www.wjgnet.com/1948-5182/full/v8/i32/1370.htm

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