Helicobacter pylori containing more phospphorylation sites of the caga protein induces greater reduction of gastric mucins

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dc.creatorMoacyr Ferreira Júnior
dc.creatorSérgio de Assis Batista
dc.creatorRafael Calvão Barbuto
dc.creatorAdriana d Gomes
dc.creatorDulciene Maria de Magalhães Queiroz
dc.creatorIvana Duval Araújo
dc.creatorMarcelo Vidigal Caliari
dc.date.accessioned2023-06-02T21:26:47Z
dc.date.accessioned2025-09-09T00:58:40Z
dc.date.available2023-06-02T21:26:47Z
dc.date.issued2017-07-10
dc.format.mimetypepdf
dc.identifier.doiDOI: 10.9734/AJMAH/2017/3461
dc.identifier.issn25468414
dc.identifier.urihttps://hdl.handle.net/1843/54421
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofAsian Journal of Medicine and Health
dc.rightsAcesso Aberto
dc.subjectHelicobacter pylori
dc.subjectMucina--1
dc.subjectMucina-5
dc.subject.otherHelicobacter pylori
dc.subject.otherMucin-1
dc.subject.otherMucin-5
dc.subject.otherCagA protein
dc.titleHelicobacter pylori containing more phospphorylation sites of the caga protein induces greater reduction of gastric mucins
dc.typeArtigo de periódico
local.citation.epage9
local.citation.issue4
local.citation.spage1
local.citation.volume5
local.description.resumoH. pylori infection is associated with gastritis, pre cancerous lesions, gastric cancer and changes in the protective layer of mucus. However, to our knowledge, there are no studies in humans or experimental models that relate the infection with H. pylori strains containing one or three phosphorylation sites of the CagA protein with the mucins of the protective layer of mucus. The aim of this study was to research the effects of infection with H. pylori strains containing one or three phosphorylation sites of CagA protein on the MUC1 and MUC5AC mucins of Mongolian gerbils (Meriones unguiculatus) gastric mucosa. Methods: Mongolian gerbils were inoculated with H. pylori isolates containing one or three EPIYA C phosphorylation sites. The control group consisted of uninfected animals receiving only Brucella broth. Gastric mucosa fragments were assessed by immunohistochemistry using rabbit monoclonal antibodies against MUC1 and MUC5AC. The fragments were then analyzed through a digital morphometric method. Results: From the control group of non-infected animals (CTRL) to the Cag A positive locci with 1 EPYIA C group (CagA1Ep) and Cag A positive locci with 3 EPYIA C group (CagA3EP) there was a progressive and significant reduction in the expression of both MUC1 (p=0.003) andMUC5AC (p=0.003) after 45 days of infection. This reduction was more prominent in the CagA3Ep group. Similar results were obtained six months post-infection, but the reduction in the MUC5AC (p=0.001) and MUC1 (p=0.001) expression was more intense. Conclusion: H. pylori strains containing more phosphorylation sites of the CagA protein are able to reduce with greater intensity the expression of MUC1 and MUC5AC.
local.identifier.orcidhttps://orcid.org/0000-0001-6488-734X
local.publisher.countryBrasil
local.publisher.departmentMED - DEPARTAMENTO DE CIRURGIA
local.publisher.departmentMED - DEPARTAMENTO DE PROPEDÊUTICA COMPLEMENTAR
local.publisher.initialsUFMG
local.url.externahttps://journalajmah.com/index.php/AJMAH/article/view/144

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