Protein Blend and Casein Supplementations before Inactive Phase Similarly Activate Mechanistic Target of Rapamycin Signaling in Rat Skeletal Muscle

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dc.creatorTales Sambrano Vieira
dc.creatorAna Paula Pinto
dc.creatorGabriela m Batitucci
dc.creatorAlisson L. Rocha
dc.creatorHugo T. Filho
dc.creatorDawit Albieiro Pinheiro Gonçalves
dc.creatorAdelino S. Silva
dc.creatorEllen Cristini de Freitas
dc.date.accessioned2024-02-27T16:19:47Z
dc.date.accessioned2025-09-08T23:25:08Z
dc.date.available2024-02-27T16:19:47Z
dc.date.issued2020
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.description.sponsorshipFAPESP - Fundação de Amparo à Pesquisa do Estado de São Paulo
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.4103/CJP.CJP_31_20
dc.identifier.issn0304-4920
dc.identifier.urihttps://hdl.handle.net/1843/64796
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofJournal of Physiological Investigation
dc.rightsAcesso Aberto
dc.subjectProteínas
dc.subjectProteínas - Pesquisa
dc.subjectProteínas - Síntese
dc.subject.otherMicellar casein
dc.subject.otherMTOR signaling pathway
dc.subject.otherProtein supplementation
dc.subject.otherProtein synthesis
dc.subject.otherSkeletal muscle
dc.subject.otherWhey protein
dc.titleProtein Blend and Casein Supplementations before Inactive Phase Similarly Activate Mechanistic Target of Rapamycin Signaling in Rat Skeletal Muscle
dc.typeArtigo de periódico
local.citation.epage178
local.citation.issue4
local.citation.spage171
local.citation.volume63
local.description.resumoDuring overnight sleep, the longest postabsorptive and inactive phase of the day causes protein catabolism and loss. However, the daytime ingestion of dairy proteins has been shown to stimulate muscle protein synthesis and growth. This study compared the effects of pre-sleep supplementation of a protein blend (PB) composed of micellar casein (MCa) and whey protein (1:1) versus isolate MCa on the plasma levels of branched-chain amino acids (BCAAs) and the activation of the mechanistic target of rapamycin (mTOR) signaling, a critical intracellular pathway involved in the regulation of muscle protein synthesis. After 10 h of fasting during the active phase, rats were fed with a single dose of PB or MCa (5.6 g protein/kg of body mass) by gavage, and samples of blood and gastrocnemius muscle were collected at 30, 90, and 450 min. PB and MCa supplementations induced an increase (~3-fold, P < 0.001) of plasma BCAAs at 30 and 90 min. Most importantly, the stimulatory phosphorylation levels of mTOR and its downstream target p70 ribosomal protein S6 kinase (p70S6K) were similarly higher (~2.5-fold, P < 0.001) 30 and 90 min after MCa and PB. Plasma levels of leucine, isoleucine, valine, and overall BCAAs were correlated with the activation of mTOR (P < 0.001) and p70S6K (P < 0.001). MCa and PB supplementations before the inactive phase of rats resulted in an anabolic milieu in the skeletal muscle by inducing a transient increase in plasma BCAAs and a similar activation of the mTOR/p70S6K axis.
local.identifier.orcidhttps://orcid.org/0000-0003-2621-3330
local.publisher.countryBrasil
local.publisher.departmentEEF - DEPARTAMENTO DE EDUCAÇÃO FÍSICA
local.publisher.departmentEEFFTO - ESCOLA DE EDUCAÇÃO FISICA, FISIOTERAPIA E TERAPIA OCUPACIONAL
local.publisher.initialsUFMG
local.url.externahttps://journals.lww.com/cjop/fulltext/2020/63040/protein_blend_and_casein_supplementations_before.4.aspx

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