Optimal cytokinin/auxin balance for indirect shoot organogenesis of Eucalyptus cloeziana and production of ex vitro rooted micro-cuttings

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dc.creatorLeandro Silva de Oliveira
dc.creatorGilvano Ebling Brondani
dc.creatorLetícia Vaz Molinari
dc.creatorRafaella Zanetti Dias
dc.creatorGustavo Leal Teixeira
dc.creatorAntônio Natal Gonçalves
dc.creatorMarcílio de Almeida
dc.date.accessioned2023-10-27T18:49:38Z
dc.date.accessioned2025-09-09T01:27:50Z
dc.date.available2023-10-27T18:49:38Z
dc.date.issued2022
dc.identifier.issn1007-662X
dc.identifier.urihttps://hdl.handle.net/1843/60177
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofJournal of Forestry Research
dc.rightsAcesso Restrito
dc.subjectPlantas - Propagação in vitro
dc.subjectPlantas - Reguladores
dc.subjectEucalipto
dc.subject.otherMicropropagation
dc.subject.otherRecalcitrant species
dc.subject.otherPlant growth regulator
dc.subject.otherExplants source
dc.titleOptimal cytokinin/auxin balance for indirect shoot organogenesis of Eucalyptus cloeziana and production of ex vitro rooted micro-cuttings
dc.typeArtigo de periódico
local.citation.epage1584
local.citation.spage1573
local.citation.volume33
local.description.resumoShoot organogenesis is critical for the shortening of long breeding cycles and circumvent the barrier of cloning mature Eucalyptus cloeziana trees. It enables large-scale production of plants from transformed tissues. This study evaluates the effect of α-naphthaleneacetic acid (NAA), thidiazuron (TDZ) and benzylaminopurine (BAP) on the organogenesis of E. cloeziana from hypocotyls and cotyledonary leaves. In the induction stage, hypocotyls and cotyledonary leaves were established in a Murashige and Skoog (MS) culture medium supplemented with NAA or TDZ. Callus tissues were cultivated in a MS culture medium containing only BAP or different concentrations of BAP/NAA in the differentiation stage. Adventitious buds were multiplied in vitro and elongated in a WPM culture medium supplemented with 0.89 μM BAP and 0.05 μM NAA. Cot- yledonary leaves exhibited the best in vitro regeneration. The induction of adventitious buds occurred only in calluses induced from TDZ. In the differentiation stage, 4.4 μM BAP treatment promoted an increase of adventitious bud regeneration. Micro-cuttings from regenerated shoots were acclimatized and rooted ex vitro in mini-incubators. The results confirm the establishment of an efficient protocol for the in vitro regeneration of E. cloeziana by indirect organogenesis, providing new insights regarding cloning of this species.
local.publisher.countryBrasil
local.publisher.departmentICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS
local.publisher.initialsUFMG
local.url.externahttps://doi.org/10.1007/s11676-022-01454-9

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