Grafting biomaterials associated to topical glucocorticoid: effects on pre-osteoblastic cells (MC3T3-E1)

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dc.creatorAlice de Araújo Ferreira Silva
dc.creatorCarolina Nemésio de Barros Pereira
dc.creatorDanilo Rocha Dias
dc.creatorFrederico Santos Lages
dc.creatorKatia Lucy Melo Maltos
dc.creatorAllyson Nogueira Moreira
dc.creatorElton Gonçalves Zenobio
dc.creatorIvana Márcia Alves Diniz
dc.date.accessioned2023-11-20T20:10:47Z
dc.date.accessioned2025-09-08T23:06:44Z
dc.date.available2023-11-20T20:10:47Z
dc.date.issued2022
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.1590/1807-3107bor-2022.vol36.0090
dc.identifier.issn1807-3107
dc.identifier.urihttps://hdl.handle.net/1843/61148
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofBrazilian Oral Research
dc.rightsAcesso Aberto
dc.subjectOdontologia
dc.subjectTransplantes
dc.subjectSubstitutos Ósseos
dc.subject.otherEnxertos
dc.subject.otherBiomateriais
dc.subject.otherBone Substitutes
dc.subject.otherHydroxyapatites
dc.subject.otherGlucocorticoids
dc.subject.otherBudesonide
dc.titleGrafting biomaterials associated to topical glucocorticoid: effects on pre-osteoblastic cells (MC3T3-E1)
dc.typeArtigo de periódico
local.citation.epage11
local.citation.spagee090
local.citation.volume36
local.description.resumoThe topical glucocorticoid budesonide has been prescribed before and after sinus lift surgery as adjuvant drug treatment for maxillary sinus membrane inflammation. However, there is no study on the effects of budesonide on the regenerative process of bone grafting biomaterials. We investigated the effect of the association of budesonide with some biomaterials on the growth and differentiation capacity of pre-osteoblastic cells (MC3T3-E1 subclone 4). Xenogeneic (Bio-Oss and Bio-Gen) and synthetic hydroxyapatites (Osteogen, Bonesynth, and HAP-91) were tested in conditioned medium (1% w/v). The conditioned medium was then supplemented with budesonide (0.5% v/v). Cell viability was assessed using the MTT assay (48, 96, and 144 h), and mineralized nodules were quantified after 14 days of culture using the Alizarin Red Staining. Alkaline phosphatase activity was assessed through the release of thymolphthalein at day seven. All biomaterials showed little or no cytotoxicity. The Bio-Gen allowed significantly less growth than the control group regardless of the experimental time. Regarding differentiation potential of MC3T3-E1, the HAP-91-conditioned medium showed remarkable osteoinductive properties. In osteodifferentiation, the addition of budesonide favored the formation of mineral nodules when cells were cultured in medium conditioned with synthetic materials, whereas it weakened the mineralization potential of cells cultured in xenogeneic medium. Regardless of whether budesonide was added or not, Osteogen and Bio-Oss showed higher alkaline phosphatase activity than the other groups. Budesonide may improve bone formation when associated with synthetic biomaterials. Conversely, the presence of this glucocorticoid weakens the mineralization potential of pre-osteoblastic cells cultured with xenogeneic hydroxyapatites.
local.identifier.orcidhttps://orcid.org/0000-0001-6229-4778
local.identifier.orcidhttps://orcid.org/0000-0001-7574-5697
local.identifier.orcidhttps://orcid.org/0000-0002-9194-8774
local.identifier.orcidhttps://orcid.org/0000-0001-7013-7043
local.identifier.orcidhttps://orcid.org/0000-0002-7022-5593
local.identifier.orcidhttps://orcid.org/0000-0002-0537-1535
local.identifier.orcidhttps://orcid.org/0000-0003-4261-0037
local.publisher.countryBrasil
local.publisher.departmentFAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORA
local.publisher.initialsUFMG
local.url.externahttps://www.scielo.br/j/bor/a/fMBvtLFt3BPszdRXhFpGXjh/?lang=en

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