Effects of three γ-alkylidene-γ-lactams on the formation of multispecies biofilms

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dc.creatorJosiane de Almeida
dc.creatorAndrea de Lima Pimenta
dc.creatorUlisses Alves Pereira
dc.creatorLuiz Cláudio de Almeida Barbosa
dc.creatorMichel Hoogenkamp
dc.creatorSuzette van der Waal
dc.creatorWim Crielaard
dc.creatorWilson Tadeu Felippe
dc.date.accessioned2023-03-08T14:44:13Z
dc.date.accessioned2025-09-08T22:58:50Z
dc.date.available2023-03-08T14:44:13Z
dc.date.issued2018-03-08
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.description.sponsorshipOutra Agência
dc.identifier.doihttps://doi.org/10.1111/eos.12411
dc.identifier.issn1600-0722
dc.identifier.urihttps://hdl.handle.net/1843/50739
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofEuropean Journal of Oral Sciences
dc.rightsAcesso Restrito
dc.subjectBiofilme
dc.subjectMatriz extracelular
dc.subject.otherlactams
dc.subject.otherBiofilm inhibition
dc.subject.otherg-alquilydenebutenolide
dc.titleEffects of three γ-alkylidene-γ-lactams on the formation of multispecies biofilms
dc.typeArtigo de periódico
local.citation.epage221
local.citation.issue3
local.citation.spage214
local.citation.volume126
local.description.resumoThis study evaluated the inhibitory effects of lactams on Streptococcus mutans, Enterococcus faecalis, and Candida glabrata multispecies biofilm formation. γ-Alkylidene-γ-lactams 1, 2, and 3 [solubilized in 3.5% dimethyl sulfoxide (DMSO)] were tested. Glass coverslips were conditioned with either the lactams or 3.5% DMSO (control) for 1 h, inoculated with microbial cultures, and incubated for 48 h. To assess the effect of the lactams on biofilm formation, the following parameters were determined: the biofilm biomass (by both crystal violet staining and protein determination); the amount of insoluble polysaccharides of the extracellular matrix; and the number of viable and total cells [by both colony-forming unit counting and quantitative real-time PCR (qPCR)]. Data were analysed using one-way anova and post-hoc Tukey tests. Lactams 1, 2, and 3 promoted a statistically significant reduction in the amount of biofilm biomass, but only lactam 3 resulted in a statistically significant reduction in the number of attached viable E. faecalis. Both total protein content and the amount of extracellular polysaccharides decreased significantly. The effects of γ-alkylidene-γ-lactams 1, 2, and 3 on the inhibition of multispecies biofilm formation were evident by their ability to reduce the amount of protein and extracellular polysaccharides.
local.publisher.countryBrasil
local.publisher.departmentICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS
local.publisher.initialsUFMG
local.url.externahttps://onlinelibrary.wiley.com/doi/10.1111/eos.12411

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