Bothrops moojeni snake peptide cyclodextrin complex: production, characterization and in vitro evaluation

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dc.creatorKarina Imaculada Rosa Teixeira
dc.creatorRobson Augusto de Souza Santos
dc.creatorFabio de Oliveira
dc.creatorRubén Dario Sinisterra Millán
dc.creatorMaría Esperanza Cortés Segura
dc.date.accessioned2023-11-28T15:53:17Z
dc.date.accessioned2025-09-08T23:31:20Z
dc.date.available2023-11-28T15:53:17Z
dc.date.issued2016
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.33263/LIANBS54.467474
dc.identifier.issn2284-6808
dc.identifier.urihttps://hdl.handle.net/1843/61450
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofLetters in Applied NanoBioScience
dc.rightsAcesso Aberto
dc.subjectCiclodextrinas
dc.subjectCarcinoma de células escamosas
dc.subjectAgentes antineoplásicos
dc.subjectPotencial zeta
dc.subjectSistemas de distribuição de medicamentos
dc.subject.otherBmooMPα-I
dc.subject.otherPeptide
dc.subject.otherHydroxypropyl-beta-cyclodextrin
dc.subject.otherHuman epidermoid carcinoma cells
dc.subject.otherSlow delivery
dc.titleBothrops moojeni snake peptide cyclodextrin complex: production, characterization and in vitro evaluation
dc.typeArtigo de periódico
local.citation.epage474
local.citation.issue4
local.citation.spage467
local.citation.volume5
local.description.resumoThe BmooMPα-I moojeni snake peptide was studied aimed to increase the therapeutic use as antitumor agent. Complexes were prepared and its physical characteristics were determined: particle size and Zeta-potential (ζ), antiproliferative and cytotoxic effects. BmooMPα-I peptide associated to Hp-βCD was able to change the cell surface parameters, such as ζ-potential which was negative. Hp-βCD:BmooMPα-I and BmooMPα-I alter the ζ of cells in contact with peptides and its association compound shown negative charge, that no favor the interaction with biological surfaces. In comparison the pure peptide as biocidal of epidermoid Carcinoma cells A431 or epithelial cells rather the fibroblast, the BmooMPα-I / Hp-βCD resulted in significant inhibition of Caco-2 human epithelial cancer cells (31.2 μg/mL after 24h), and Human Epidermoid Carcinoma cells A431(3.9 μg/mL after 24h), in a more efficient manner than peptide alone (p< 0.001), and was able to alter the degree of hemolysis. Next, the Lactate Dehydrogenase was examined to know the peptide cytotoxic effects and showed lower cytotoxic effects on EC50 for fibroblastic cells. Based on the BmooMPα-I /Hp-βCD properties observed and its ability of inhibiting the proliferation of epithelial cells, this study highlight that this compound is a biocidal with high potential as an anti-tumoral agent.
local.identifier.orcidhttps://orcid.org/0000-0002-0560-8491
local.identifier.orcidhttps://orcid.org/0000-0001-7656-1849
local.publisher.countryBrasil
local.publisher.departmentFAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORA
local.publisher.departmentICB - DEPARTAMENTO DE FISIOLOGIA E BIOFÍSICA
local.publisher.departmentICX - DEPARTAMENTO DE QUÍMICA
local.publisher.initialsUFMG
local.url.externahttps://nanobioletters.com/wp-content/uploads/2021/02/2284680853467474.pdf

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