Short-term evaluation of photobiomodulation therapy on the proliferation and undifferentiated status of dental pulp stem cells

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dc.creatorLeila Soares Ferreira
dc.creatorIvana Márcia Alves Diniz
dc.creatorCarlos Maranduba
dc.creatorSueli Patrícia Harumi Miyiagi
dc.creatorMaria Fernanda Rodrigues
dc.creatorCácio Moura-Neto
dc.creatorMárcia Martins Marques
dc.date.accessioned2022-12-18T00:49:49Z
dc.date.accessioned2025-09-08T23:58:20Z
dc.date.available2022-12-18T00:49:49Z
dc.date.issued2018-09-24
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPESP - Fundação de Amparo à Pesquisa do Estado de São Paulo
dc.identifier.doihttps://doi.org/10.1007/s10103-018-2637-z
dc.identifier.issn1435-604X
dc.identifier.urihttps://hdl.handle.net/1843/48171
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofLasers in Medical Science
dc.rightsAcesso Restrito
dc.subjectLasers semiconductor
dc.subjectGene expression
dc.subjectMesenchymal stem cells
dc.subjectCell proliferation
dc.subject.otherCell proliferation
dc.titleShort-term evaluation of photobiomodulation therapy on the proliferation and undifferentiated status of dental pulp stem cells
dc.typeArtigo de periódico
local.citation.epage666
local.citation.spage659
local.citation.volume34
local.description.resumoThe aim of this in vitro study was to analyze the effect of photobiomodulation therapy (PBMT) on the proliferation and undifferentiating status of stem cell from human exfoliated deciduous teeth (SHEDs). PBMT was carried out with an aluminum gallium indium phosphide (InGaAlP) diode laser in contact and punctual mode (continuous wave, 660 nm, 20 mW, 0.028 cm2, and average energy densities of 1 (1 s), 3 (4 s), 5 (7 s), 10 (14 s), 15 (21 s), or 20 (28 s) J/cm2 per point). The immunoprofile of the SHEDs was analyzed using flow cytometry. Cell proliferation was assessed by the MTT reduction assay. Gene expressions of mesenchymal stem cell markers (OCT4, Nestin, CD90, and CD105) were assessed by RT-qPCR 48 h after PBMT. Data were compared by analysis of variance (ANOVA) and Tukey's test (p ≤ 0.05). Cells cultured under nutritional deficit and treated with PBMT at 5 J/cm2 presented similar cell growth than those of positive control group. Cell growth was significantly higher than those of other groups. Mesenchymal stem cell gene markers were still expressed after PBMT at 5 J/cm2. In a short-term analysis, PBMT increases the number of stem cells with no interference in the undifferentiated state of the irradiated cells, which opens wide possibilities for application in tissue regeneration.
local.publisher.countryBrasil
local.publisher.departmentFAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORA
local.publisher.initialsUFMG
local.url.externahttps://link.springer.com/article/10.1007/s10103-018-2637-z

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