Probiotic Propionibacterium freudenreichii requires SlpB protein to mitigate mucositis induced by chemotherapy

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dc.creatorFillipe Luiz Rosado Carmo
dc.creatorHouem Rabah
dc.creatorBarbara Fernandes Cordeiro
dc.creatorSara Heloisa da Silva
dc.creatorRafaela Miranda Pessoa
dc.creatorSimone Odília Antunes Fernandes
dc.creatorValbert Nascimento Cardoso
dc.creatorValérie Gagnaire
dc.creatorMartine Deplanche
dc.creatorBruna Savassi
dc.creatorAlessandra Figueiroa
dc.creatorEmiliano Rosa Oliveira
dc.creatorCaio César Fonseca
dc.creatorMaria Izabel Alves Queiroz
dc.creatorNúbia Moraes Rodrigues
dc.creatorSávio Henrique de Cicco Sandes
dc.creatorÁlvaro Cantini Nunes
dc.creatorLuisa Lemos
dc.creatorJuliana de Lima Alves
dc.creatorAna Maria Caetano Faria
dc.creatorÊnio Ferreira
dc.creatorYves Le Loir
dc.creatorGwénaël Jan
dc.creatorVasco Azevedo
dc.date.accessioned2022-04-06T22:39:21Z
dc.date.accessioned2025-09-09T00:18:46Z
dc.date.available2022-04-06T22:39:21Z
dc.date.issued2020
dc.description.sponsorshipOutra Agência
dc.format.mimetypepdf
dc.identifier.doi10.18632/oncotarget.27319
dc.identifier.issn1949-2553
dc.identifier.urihttps://hdl.handle.net/1843/40862
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofOncotarget
dc.rightsAcesso Aberto
dc.subjectProbiótico
dc.subjectMucosite
dc.subjectQuimioterapia
dc.subject.otherProbiotic
dc.subject.otherMucositis
dc.subject.otherSurface protein
dc.subject.otherImmunomodulation
dc.subject.otherInflammation
dc.titleProbiotic Propionibacterium freudenreichii requires SlpB protein to mitigate mucositis induced by chemotherapy
dc.typeArtigo de periódico
local.citation.epage7219
local.citation.issue68
local.citation.spage7198
local.citation.volume10
local.description.resumoPropionibacterium freudenreichii CIRM-BIA 129 (P. freudenreichii wild type, WT) is a probiotic bacterium, which exerts immunomodulatory effects. This strain possesses extractable surface proteins, including SlpB, which are involved in anti-inflammatory effect and in adhesion to epithelial cells. We decided to investigate the impact of slpB gene mutation on immunomodulation in vitro and in vivo. In an in vitro assay, P. freudenreichii WT reduced expression of IL-8 (p<0.0001) and TNF-α (p<0.0001) cytokines in LPS-stimulated HT-29 cells. P. freudenreichii ΔslpB, lacking the SlpB protein, failed to do so. Subsequently, both strains were investigated in vivo in a 5-FU-induced mucositis mice model. Mucositis is a common side effect of cytotoxic chemotherapy with 5-FU, characterized by mucosal injury, inflammation, diarrhea, and weight loss. The WT strain prevented weight loss, reduced inflammation and consequently histopathological scores. Furthermore, it regulated key markers, including Claudin-1 (cld1, p<0.0005) and IL-17a (Il17a, p<0.0001) genes, as well as IL-12 (p<0.0001) and IL-1β (p<0.0429) cytokines levels. Mutant strain displayed opposite regulatory effect on cld1 expression and on IL-12 levels. This work emphasizes the importance of SlpB in P. freudenreichii ability to reduce mucositis inflammation. It opens perspectives for the development of probiotic products to decrease side effects of chemotherapy using GRAS bacteria with immunomodulatory surface protein properties.
local.publisher.countryBrasil
local.publisher.departmentFAR - DEPARTAMENTO DE ALIMENTOS
local.publisher.departmentFAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICAS
local.publisher.departmentICB - DEPARTAMENTO DE BIOQUÍMICA E IMUNOLOGIA
local.publisher.initialsUFMG
local.url.externahttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6944450/

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