Low doses of pharmaceutical formulations loaded with UFMG-V4N2 immunogen induce the production of IgG anti-cocaine antibodies and provide evidence of cerebral protection in the preclinical model

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dc.creatorBruna Rodrigues Dias Assis
dc.creatorValbert Nascimento Cardoso
dc.creatorÂngelo de Fátima
dc.creatorFrederico Duarte Garcia
dc.creatorGisele Assis Castro Goulart
dc.creatorMaila de Castro Lourenço das Neves
dc.creatorPaulo Sérgio de Almeida Augusto
dc.creatorRaissa Lima Gonçalves Pereira
dc.creatorSordaini Maria Caligiorni
dc.creatorBrian Sabato
dc.creatorLarissa Pires do Espírito Santo
dc.creatorKarine Dias dos Reis
dc.creatorLeonardo da Silva Neto
dc.creatorSimone Odilia Antunes Fernandes
dc.date.accessioned2024-11-13T23:59:20Z
dc.date.accessioned2025-09-09T00:53:28Z
dc.date.available2024-11-13T23:59:20Z
dc.date.issued2023
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipOutra Agência
dc.format.mimetypepdf
dc.identifier.doiHttps://doi.org/10.1016/j.jciso.2023.100078
dc.identifier.issn2666-934X
dc.identifier.urihttps://hdl.handle.net/1843/78034
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofJCIS Open
dc.rightsAcesso Aberto
dc.subjectCalixarenos
dc.subjectQuímica farmacêutica
dc.subjectCocaína
dc.subjectVacinas
dc.subject.otherUFMG-V4N2
dc.subject.otherCalix[4]arene-based immunogen
dc.subject.otherPharmaceutical formulation
dc.subject.otherAnti-cocaine therapy
dc.titleLow doses of pharmaceutical formulations loaded with UFMG-V4N2 immunogen induce the production of IgG anti-cocaine antibodies and provide evidence of cerebral protection in the preclinical model
dc.typeArtigo de periódico
local.citation.spage100078
local.citation.volume9
local.description.resumoAnti-cocaine vaccines are a promising therapeutic strategy for treating cocaine use disorders. Here we hypothesize that nanoemulsions (NE) or suspensions (SS) loaded with the calix [4]arene-based immunogen UFMG-V4N2 can induce the production of anti-cocaine antibodies and decrease the passage of radiolabeled cocaine analog [99mTc]Trodat-1 through of the brain barrier. UFMG-V4N2 was characterized (solubility, morphology, DSC, XRD) and loaded into NEs and SSs using excipients approved for human use. Immunogenic efficacy was assessed by quantifying the titers and determining the specificity of anti-cocaine IgG antibodies, and by assessing the inhibition of [99mTc]Trodat-1 trafficking across the mice brain-barrier. UFMG-V4N2 is an amorphous, thermally stable molecule with very low hydrophilicity. The immunogenicity of NE or SS was similar, but aluminum phosphate and the lower dose of UFMG-V4N2 induced higher anti-cocaine IgG antibody titers, minimizing [99mTc]Trodat-1 uptake in the brain. Therefore, the UFMG-V4N2 may represent an alternative for the treatment of cocaine use disorder.
local.identifier.orcidhttps://orcid.org/0000-0001-7597-9602
local.identifier.orcidhttps://orcid.org/0000-0003-2344-5590
local.identifier.orcidhttps://orcid.org/0000-0003-1926-9053
local.identifier.orcidhttps://orcid.org/0000-0003-0292-076X
local.identifier.orcidhttps://orcid.org/0000-0003-4125-3736
local.identifier.orcidhttps://orcid.org/0000-0002-2303-4452
local.identifier.orcidhttps://orcid.org/0000-0002-4154-4237
local.identifier.orcidhttps://orcid.org/0000-0003-0825-7604
local.identifier.orcidhttps://orcid.org/0000-0001-7830-9882
local.identifier.orcidhttps://orcid.org/0000-0003-4554-9577
local.identifier.orcidhttps://orcid.org/0000-0002-6139-5187
local.publisher.countryBrasil
local.publisher.departmentICX - DEPARTAMENTO DE QUÍMICA
local.publisher.departmentMED - DEPARTAMENTO DE SAÚDE MENTAL
local.publisher.initialsUFMG
local.url.externahttps://www.sciencedirect.com/science/article/pii/S2666934X23000053?via%3Dihub

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