Urocortin 2 promotes hypertrophy and enhances skeletal muscle function through cAMP and insulin/IGF-1 signaling pathways

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dc.creatorNatalia Lautherbach
dc.creatorIsis C. Kettelhut
dc.creatorDawit Albieiro Pinheiro Gonçalves
dc.creatorWilian A. Silveira
dc.creatorSílvia Paula-Gomes
dc.creatorRafael Rossi Valentim
dc.creatorNeuza M. Zanon
dc.creatorMarcelo G. Pereira
dc.creatorElen H. Miyabara
dc.creatorLuiz C. C. Navegantes
dc.date.accessioned2024-02-27T16:22:11Z
dc.date.accessioned2025-09-09T00:40:26Z
dc.date.available2024-02-27T16:22:11Z
dc.date.issued2022
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.description.sponsorshipFAPESP - Fundação de Amparo à Pesquisa do Estado de São Paulo
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.1016/j.molmet.2022.101492
dc.identifier.issn2212-8778
dc.identifier.urihttps://hdl.handle.net/1843/64803
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofMolecular Metabolism
dc.rightsAcesso Aberto
dc.subjectHipertrofia
dc.subjectFadiga
dc.subjectProteínas
dc.subject.otherUrocortin 2
dc.subject.otherHypertrophy
dc.subject.otherFatigue resistance
dc.subject.othercAMP
dc.titleUrocortin 2 promotes hypertrophy and enhances skeletal muscle function through cAMP and insulin/IGF-1 signaling pathways
dc.typeArtigo de periódico
local.citation.epage16
local.citation.spage101492
local.citation.volume60
local.description.resumoObjective Although it is well established that urocortin 2 (Ucn2), a peptide member of the corticotrophin releasing factor (CRF) family, and its specific corticotrophin-releasing factor 2 receptor (CRF2R) are highly expressed in skeletal muscle, the role of this peptide in the regulation of skeletal muscle mass and protein metabolism remains elusive. Methods To elucidate the mechanisms how Ucn2 directly controls protein metabolism in skeletal muscles of normal mice, we carried out genetic tools, physiological and molecular analyses of muscles in vivo and in vitro. Results Here, we demonstrated that Ucn2 overexpression activated cAMP signaling and promoted an expressive muscle hypertrophy associated with higher rates of protein synthesis and activation of Akt/mTOR and ERK1/2 signaling pathways. Furthermore, Ucn2 induced a decrease in mRNA levels of atrogin-1 and in autophagic flux inferred by an increase in the protein content of LC3-I, LC3-II and p62. Accordingly, Ucn2 reduced both the transcriptional activity of FoxO in vivo and the overall protein degradation in vitro through an inhibition of lysosomal proteolytic activity. In addition, we demonstrated that Ucn2 induced a fast-to-slow fiber type shift and improved fatigue muscle resistance, an effect that was completely blocked in muscles co-transfected with mitogen-activated protein kinase phosphatase 1 (MKP-1), but not with dominant-negative Akt mutant (Aktmt). Conclusions These data suggest that Ucn2 triggers an anabolic and anti-catabolic response in skeletal muscle of normal mice probably through the activation of cAMP cascade and participation of Akt and ERK1/2 signaling. These findings open new perspectives in the development of therapeutic strategies to cope with the loss of muscle mass.
local.identifier.orcidhttps://orcid.org/0000-0003-2621-3330
local.publisher.countryBrasil
local.publisher.departmentEEF - DEPARTAMENTO DE EDUCAÇÃO FÍSICA
local.publisher.departmentEEFFTO - ESCOLA DE EDUCAÇÃO FISICA, FISIOTERAPIA E TERAPIA OCUPACIONAL
local.publisher.initialsUFMG
local.url.externahttps://www.sciencedirect.com/science/article/pii/S2212877822000618?via%3Dihub

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