HIF-1α is overexpressed in odontogenic keratocyst suggesting activation of HIF-1α and notch1 signaling pathways

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dc.creatorNatacha Malu Miranda da Costa
dc.creatorJoão de Jesus Viana Pinheiro
dc.creatorCaio Tadashi Saab Abe
dc.creatorGeovanni Pereira Mitre
dc.creatorRicardo Alves de Mesquita
dc.creatorMaria Sueli da Silva Kataoka
dc.creatorAndré Luis Ribeiro Ribeiro
dc.creatorRuy Gastaldoni Jaeger
dc.creatorSérgio de Melo Alves-Júnior
dc.creatorAndrew Mark Smith
dc.date.accessioned2024-10-23T21:02:13Z
dc.date.accessioned2025-09-08T22:52:59Z
dc.date.available2024-10-23T21:02:13Z
dc.date.issued2019-07-17
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.3390/cells8070731
dc.identifier.issn2073-4409
dc.identifier.urihttps://hdl.handle.net/1843/77613
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofCells
dc.rightsAcesso Aberto
dc.subjectOdontogenic cysts
dc.subjectHypoxia
dc.subjectOxygen
dc.subjectImmunohistochemistry
dc.subjectProteins
dc.subjectMouth mucosa
dc.subjectOdontogenic cyst, calcifying
dc.subjectHypoxia-inducible factor 1, alpha subunit
dc.subjectReceptors, notch
dc.subjectDisintegrins
dc.subjectMetalloproteases
dc.subjectADAM12 protein
dc.subjectEpidermal growth factor
dc.subjectHeparin
dc.subjectEpithelium
dc.subject.otherOdontogenic keratocyst
dc.subject.otherHIF-1α
dc.subject.otherHypoxia
dc.subject.otherOxygen
dc.titleHIF-1α is overexpressed in odontogenic keratocyst suggesting activation of HIF-1α and notch1 signaling pathways
dc.typeArtigo de periódico
local.citation.epage744
local.citation.issue7
local.citation.spage731
local.citation.volume8
local.description.resumoBackground: The odontogenic keratocyst (OKC) is an odontogenic cyst that shows aggressive and intriguing biological behavior. It is suggested that a hypoxic environment occurs in OKC, which led us to investigate the immunoexpression and location of hypoxia-inducible factor 1-alpha (HIF-1α) and other hypoxia-related proteins. Methods: Twenty cases of OKC were evaluated for the expression of notch homolog 1 (notch1), HIF-1α, disintegrin and metalloproteinase domain-containing protein 12 (ADAM-12), and heparin-binding epidermal growth factor-like growth factor (HBEGF) by immunohistochemistry and compared to eight control cases of calcifying odontogenic cystic (COC), orthokeratinized odontogenic cyst (OOC), and normal oral mucosa (OM) in basal and parabasal layers. Results: In OKC, all the proteins tested were expressed significantly higher in both basal (except for notch1 and HBEGF in OOC) and suprabasal epithelial layers compared to controls. Looking at the epithelial layers within OKC, we observed an increased notch1 and HIF-1α expression in parabasal layers. Conclusions: These results suggest that hypoxia occurs more intensively in OKC compared to COC, OM, and OOC. Hypoxia appeared to be stronger in parabasal layers as observed by higher HIF-1α expression in upper cells. Overexpression of notch1, ADAM-12, and HBEGF in OKC was observed, which suggests that microenvironmental hypoxia could potentially regulate the expression of hypoxia-related proteins, and consequently, its clinical and biological behavior.
local.identifier.orcidhttps://orcid.org/0000-0001-7852-4697
local.identifier.orcidhttps://orcid.org/0000-0002-6914-6441
local.identifier.orcidhttps://orcid.org/0000-0001-7011-0457
local.identifier.orcidhttps://orcid.org/0000-0003-3207-4007
local.identifier.orcidhttps://orcid.org/0000-0001-9650-1034
local.identifier.orcidhttps://orcid.org/0000-0001-8124-2156
local.identifier.orcidhttps://orcid.org/0000-0002-1564-0356
local.identifier.orcidhttps://orcid.org/0000-0003-0272-9279
local.identifier.orcidhttps://orcid.org/0000-0002-4691-5973
local.publisher.countryBrasil
local.publisher.departmentFAO - DEPARTAMENTO DE CLÍNICA
local.publisher.initialsUFMG
local.url.externahttps://www.mdpi.com/2073-4409/8/7/731

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