Hypoxia reduces the E-cadherin expression and increases OSCC cell migration regardless of the E-cadherin methylation profile

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dc.creatorPatrícia Luciana Batista Domingos
dc.creatorMarcela Gonçalves Souza
dc.creatorTalita Antunes Guimarães
dc.creatorEliane Sobrinho Santos
dc.creatorLucyana Conceição Farias
dc.creatorCarlos Alberto de Carvalho Fraga
dc.creatorKimberly Marie Jones
dc.creatorSérgio Henrique Sousa Santos
dc.creatorAlfredo Maurício Batista de Paula
dc.creatorAndré Luiz Sena Guimarães
dc.date.accessioned2022-08-18T13:17:37Z
dc.date.accessioned2025-09-08T22:51:13Z
dc.date.available2022-08-18T13:17:37Z
dc.date.issued2017-05
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.identifier.doihttps://doi.org/10.1016/j.prp.2017.02.003
dc.identifier.issn0344-0338
dc.identifier.urihttps://hdl.handle.net/1843/44355
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofPathology: Research and Practice
dc.rightsAcesso Aberto
dc.subjectBoca - Câncer
dc.subjectGlicoproteínas
dc.subjectMigração celular
dc.subjectTumores
dc.subjectCultura de células
dc.titleHypoxia reduces the E-cadherin expression and increases OSCC cell migration regardless of the E-cadherin methylation profile
dc.typeArtigo de periódico
local.citation.epage501
local.citation.issue5
local.citation.spage496
local.citation.volume213
local.description.resumoObjective: the purpose of the current study is to investigate the association between E-cadherin methylation status, hypoxia and OSCC. Methods: HaCat and SCC9 cell lines were submitted to hypoxic treatment, followed by methylation profile analysis (MS-PCR) and analysis of the expression of mRNA gene E-cadherin (RT-PCR). Study group samples comprise individuals affected by potentially malignant lesions Potential Malignant Oral Lesion (PMOL, n = 18) and oral squamous cell carcinoma (OSCC, n = 28). The control group oral mucosa (OM, n = 15) of patients with an oral mucocele. Cell migration ability was evaluated a scratch wound assay in SCC9 and HaCat cell lines. Results: E-cadherin mRNA expression in the cell lines SCC9 and HaCat was significantly reduced under hypoxia, regardless of the methylation profile, when compared to the control group. No differences in methylation profile of the E-cadherin were observed among the groups OM, PMOL and OSCC. HaCat and SCC9 presented increases in cell migration rates under hypoxia. Conclusion: the current study demonstrates that hypoxia reduces E-cadherin expression and increase cell migration, regardless of the methylation profile. Additionally, no differences in E-cadherin methylation patterns were observed among OM, PMOL and OSCC.
local.publisher.countryBrasil
local.publisher.departmentICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS
local.publisher.initialsUFMG
local.url.externahttps://www.sciencedirect.com/science/article/pii/S0344033816303004

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