Liberação de cortisol sérico durante a expansão rápida da maxila : validação de modelo experimental

Abstract

Introduction: Rapid maxillary expansion (REM) is an orthopedic treatment for correcting maxillary constriction in the transverse direction through forces applied by devices. This procedure causes dental and skeletal changes that can trigger stress. Objective: To validate the maxillary ERM model, using cortisol levels in the serum of mice to estimate stress during the disjunction procedure. Methods: Male mice (C57BL6/J) 5 to 6 weeks old underwent MRE performed via an opening loop switch on the midpalatal suture calibrated as follows (n = 5 per group): no force (control /fake); 0.28 Newtons (N), 0.42 N and 0.56 N for periods of 7 and 14 days. Histomorphometric analyzes were performed and serum samples were collected for cortisol measurement by enzyme-linked immunosorbent assay (ELISA). Results: The three forces applied at two different times resulted in a pattern similar to the ERM. The force of 0.42 N over 7 days resulted in a significant increase (P>0.05) in cortisol (pg/ml) when compared to the control and 0.28 N groups over 7 days; the cortisol level in the 0.42 N 14 days group was statistically slow when compared to the 0.42 N 14 days group. There was no statistically significant difference in other groups. Conclusion: The results suggest that there is an increase in the stress response during the first days after the application of RME force with a force of 0.42 N, with subsequent body adaptation. The force parameter of 0.42 N over 7 days proved to be a valid model for analyzing ERM in mice.